Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain

Diffuse optical remission spectra from the mammalian neocortex at visible wavelengths contain spectral features originating from the mitochondria. A new algorithm is presented, based on analytically relating the first differential of the attenuation spectrum to the first differential of the chromoph...

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Detalles Bibliográficos
Autores principales: Sakata, Y., Abajian, M., Ripple, M. O., Springett, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Spectroscopic Diagnostics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409711/
https://www.ncbi.nlm.nih.gov/pubmed/22876356
http://dx.doi.org/10.1364/BOE.3.001933
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author Sakata, Y.
Abajian, M.
Ripple, M. O.
Springett, R.
author_facet Sakata, Y.
Abajian, M.
Ripple, M. O.
Springett, R.
author_sort Sakata, Y.
collection PubMed
description Diffuse optical remission spectra from the mammalian neocortex at visible wavelengths contain spectral features originating from the mitochondria. A new algorithm is presented, based on analytically relating the first differential of the attenuation spectrum to the first differential of the chromophore spectra, that can separate and calculate the oxidation state of cytochrome c as well as the absolute concentration and saturation of hemoglobin. The algorithm is validated in phantoms and then tested on the neocortex of the rat during an anoxic challenge. Implementation of the algorithm will provide detailed information of mitochondrial oxygenation and mitochondrial function in physiological studies of the mammalian brain.
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spelling pubmed-34097112012-08-08 Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain Sakata, Y. Abajian, M. Ripple, M. O. Springett, R. Biomed Opt Express Spectroscopic Diagnostics Diffuse optical remission spectra from the mammalian neocortex at visible wavelengths contain spectral features originating from the mitochondria. A new algorithm is presented, based on analytically relating the first differential of the attenuation spectrum to the first differential of the chromophore spectra, that can separate and calculate the oxidation state of cytochrome c as well as the absolute concentration and saturation of hemoglobin. The algorithm is validated in phantoms and then tested on the neocortex of the rat during an anoxic challenge. Implementation of the algorithm will provide detailed information of mitochondrial oxygenation and mitochondrial function in physiological studies of the mammalian brain. Optical Society of America 2012-07-25 /pmc/articles/PMC3409711/ /pubmed/22876356 http://dx.doi.org/10.1364/BOE.3.001933 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Spectroscopic Diagnostics
Sakata, Y.
Abajian, M.
Ripple, M. O.
Springett, R.
Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
title Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
title_full Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
title_fullStr Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
title_full_unstemmed Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
title_short Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
title_sort measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain
topic Spectroscopic Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409711/
https://www.ncbi.nlm.nih.gov/pubmed/22876356
http://dx.doi.org/10.1364/BOE.3.001933
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