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Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16

BACKGROUND: Probiotic lactic acid bacteria (LAB) support a functional and balanced immune system, and contribute to immune modulatory effects in combatting microbial pathogens, including viruses. Most cervical cancers are associated with anogenital region infection with high-risk (HR) human papillom...

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Autores principales: Cha, Min-Kyeong, Lee, Do-Kyung, An, Hyang-Mi, Lee, Si-Won, Shin, Seon-Hee, Kwon, Jeong-Hyun, Kim, Kyung-Jae, Ha, Nam-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409845/
https://www.ncbi.nlm.nih.gov/pubmed/22788922
http://dx.doi.org/10.1186/1741-7015-10-72
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author Cha, Min-Kyeong
Lee, Do-Kyung
An, Hyang-Mi
Lee, Si-Won
Shin, Seon-Hee
Kwon, Jeong-Hyun
Kim, Kyung-Jae
Ha, Nam-Joo
author_facet Cha, Min-Kyeong
Lee, Do-Kyung
An, Hyang-Mi
Lee, Si-Won
Shin, Seon-Hee
Kwon, Jeong-Hyun
Kim, Kyung-Jae
Ha, Nam-Joo
author_sort Cha, Min-Kyeong
collection PubMed
description BACKGROUND: Probiotic lactic acid bacteria (LAB) support a functional and balanced immune system, and contribute to immune modulatory effects in combatting microbial pathogens, including viruses. Most cervical cancers are associated with anogenital region infection with high-risk (HR) human papillomavirus (HPV). In this study, we analyzed the antiviral activity of Bifidobacterium adolescentis SPM1005-A in the SiHa cervical cancer cell line expressing HPV type 16. METHODS: We assessed the cellular toxicity of B. adolescentis SPM1005-A in SiHa cells by the Trypan blue dye exclusion assay. Cells (3.6 × 10(5)) in culture plates with or without B. adolescentis SPM1005-A in the same type of medium, were incubated with HPV type 16 at a concentration of 5.1 × 10(7 )cfu/ml. For antiviral analysis, we performed quantitative real-time PCR (qRT-PCR) for E6 and E7 oncogene expressions and observed protein levels by immunoblotting. RESULTS: The qRT-PCR results showed that E6 and E7 mRNA levels decreased simultaneously. Western blot analysis revealed that the E6 protein expression slightly decreased after 24 and 48 h, but the level of E7 protein expression appear unaffected compared with that in the control. Decreased HPV16 E6 and E7 mRNA transcript and protein levels were not associated with cell morphology or significant cytotoxic effects. CONCLUSIONS: This study showed that B. adolescentis SPM1005-A had antiviral activity through suppression E6 and E7 oncogene expression. The results suggest that B. adolescentis SPM1005-A could be potential applications of HPV-associated cervical cancer prevention.
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spelling pubmed-34098452012-08-02 Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16 Cha, Min-Kyeong Lee, Do-Kyung An, Hyang-Mi Lee, Si-Won Shin, Seon-Hee Kwon, Jeong-Hyun Kim, Kyung-Jae Ha, Nam-Joo BMC Med Research Article BACKGROUND: Probiotic lactic acid bacteria (LAB) support a functional and balanced immune system, and contribute to immune modulatory effects in combatting microbial pathogens, including viruses. Most cervical cancers are associated with anogenital region infection with high-risk (HR) human papillomavirus (HPV). In this study, we analyzed the antiviral activity of Bifidobacterium adolescentis SPM1005-A in the SiHa cervical cancer cell line expressing HPV type 16. METHODS: We assessed the cellular toxicity of B. adolescentis SPM1005-A in SiHa cells by the Trypan blue dye exclusion assay. Cells (3.6 × 10(5)) in culture plates with or without B. adolescentis SPM1005-A in the same type of medium, were incubated with HPV type 16 at a concentration of 5.1 × 10(7 )cfu/ml. For antiviral analysis, we performed quantitative real-time PCR (qRT-PCR) for E6 and E7 oncogene expressions and observed protein levels by immunoblotting. RESULTS: The qRT-PCR results showed that E6 and E7 mRNA levels decreased simultaneously. Western blot analysis revealed that the E6 protein expression slightly decreased after 24 and 48 h, but the level of E7 protein expression appear unaffected compared with that in the control. Decreased HPV16 E6 and E7 mRNA transcript and protein levels were not associated with cell morphology or significant cytotoxic effects. CONCLUSIONS: This study showed that B. adolescentis SPM1005-A had antiviral activity through suppression E6 and E7 oncogene expression. The results suggest that B. adolescentis SPM1005-A could be potential applications of HPV-associated cervical cancer prevention. BioMed Central 2012-07-12 /pmc/articles/PMC3409845/ /pubmed/22788922 http://dx.doi.org/10.1186/1741-7015-10-72 Text en Copyright ©2012 Cha et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cha, Min-Kyeong
Lee, Do-Kyung
An, Hyang-Mi
Lee, Si-Won
Shin, Seon-Hee
Kwon, Jeong-Hyun
Kim, Kyung-Jae
Ha, Nam-Joo
Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16
title Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16
title_full Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16
title_fullStr Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16
title_full_unstemmed Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16
title_short Antiviral activity of Bifidobacterium adolescentis SPM1005-A on human papillomavirus type 16
title_sort antiviral activity of bifidobacterium adolescentis spm1005-a on human papillomavirus type 16
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409845/
https://www.ncbi.nlm.nih.gov/pubmed/22788922
http://dx.doi.org/10.1186/1741-7015-10-72
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