A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis

Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized a high-throughput, liquid media-based assay to screen transposon disruptants of the phytopathogen Pseudomonas syringae pv. maculicola ES4326 to identify genes required...

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Autores principales: Schreiber, Karl J., Ye, David, Fich, Eric, Jian, Allen, Lo, Timothy, Desveaux, Darrell
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409859/
https://www.ncbi.nlm.nih.gov/pubmed/22870224
http://dx.doi.org/10.1371/journal.pone.0041461
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author Schreiber, Karl J.
Ye, David
Fich, Eric
Jian, Allen
Lo, Timothy
Desveaux, Darrell
author_facet Schreiber, Karl J.
Ye, David
Fich, Eric
Jian, Allen
Lo, Timothy
Desveaux, Darrell
author_sort Schreiber, Karl J.
collection PubMed
description Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized a high-throughput, liquid media-based assay to screen transposon disruptants of the phytopathogen Pseudomonas syringae pv. maculicola ES4326 to identify genes required for virulence on Arabidopsis. Many genes identified through this screen were involved in processes such as type III secretion, periplasmic glucan biosynthesis, flagellar motility, and amino acid biosynthesis. A small set of genes did not fall into any of these functional groups, and their disruption resulted in context-specific effects on in planta bacterial growth.
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spelling pubmed-34098592012-08-06 A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis Schreiber, Karl J. Ye, David Fich, Eric Jian, Allen Lo, Timothy Desveaux, Darrell PLoS One Research Article Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized a high-throughput, liquid media-based assay to screen transposon disruptants of the phytopathogen Pseudomonas syringae pv. maculicola ES4326 to identify genes required for virulence on Arabidopsis. Many genes identified through this screen were involved in processes such as type III secretion, periplasmic glucan biosynthesis, flagellar motility, and amino acid biosynthesis. A small set of genes did not fall into any of these functional groups, and their disruption resulted in context-specific effects on in planta bacterial growth. Public Library of Science 2012-08-01 /pmc/articles/PMC3409859/ /pubmed/22870224 http://dx.doi.org/10.1371/journal.pone.0041461 Text en © 2012 Schreiber et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schreiber, Karl J.
Ye, David
Fich, Eric
Jian, Allen
Lo, Timothy
Desveaux, Darrell
A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
title A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
title_full A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
title_fullStr A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
title_full_unstemmed A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
title_short A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis
title_sort high-throughput forward genetic screen identifies genes required for virulence of pseudomonas syringae pv. maculicola es4326 on arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409859/
https://www.ncbi.nlm.nih.gov/pubmed/22870224
http://dx.doi.org/10.1371/journal.pone.0041461
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