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A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis

In yeasts and worms, KASH (Klarsicht/ANC-1/Syne/homology) domain and SUN (Sad-1/UNC-84) domain nuclear envelope (NE) proteins play a crucial role in meiotic chromosome movement and homologue pairing. However, although the vertebrate SUN domain protein SUN1 is involved in these processes, its partner...

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Autores principales: Morimoto, Akihiro, Shibuya, Hiroki, Zhu, Xiaoqiang, Kim, Jihye, Ishiguro, Kei-ichiro, Han, Min, Watanabe, Yoshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410425/
https://www.ncbi.nlm.nih.gov/pubmed/22826121
http://dx.doi.org/10.1083/jcb.201204085
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author Morimoto, Akihiro
Shibuya, Hiroki
Zhu, Xiaoqiang
Kim, Jihye
Ishiguro, Kei-ichiro
Han, Min
Watanabe, Yoshinori
author_facet Morimoto, Akihiro
Shibuya, Hiroki
Zhu, Xiaoqiang
Kim, Jihye
Ishiguro, Kei-ichiro
Han, Min
Watanabe, Yoshinori
author_sort Morimoto, Akihiro
collection PubMed
description In yeasts and worms, KASH (Klarsicht/ANC-1/Syne/homology) domain and SUN (Sad-1/UNC-84) domain nuclear envelope (NE) proteins play a crucial role in meiotic chromosome movement and homologue pairing. However, although the vertebrate SUN domain protein SUN1 is involved in these processes, its partner has remained identified. Based on subcellular localization screening in mouse spermatocytes, we identified a novel germ cell–specific protein, KASH5, that localized exclusively at telomeres from the leptotene to diplotene stages in both spermatocytes and oocytes. KASH5 possesses hitherto unknown KASH-related sequences that directly interacted with SUN1 and mediated telomere localization. Thus, KASH5 is a mammalian meiosis-specific KASH domain protein. We show that meiotic chromosome movement depended on microtubules and that KASH5 interacted with the microtubule-associated dynein–dynactin complex. These results suggest that KASH5 connects the telomere-associated SUN1 protein to the cytoplasmic force–generating mechanism involved in meiotic chromosome movement. Our study strongly suggests that the meiotic homologue-pairing mechanism mediated by the SUN–KASH NE bridge is highly conserved among eukaryotes.
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spelling pubmed-34104252013-01-23 A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis Morimoto, Akihiro Shibuya, Hiroki Zhu, Xiaoqiang Kim, Jihye Ishiguro, Kei-ichiro Han, Min Watanabe, Yoshinori J Cell Biol Research Articles In yeasts and worms, KASH (Klarsicht/ANC-1/Syne/homology) domain and SUN (Sad-1/UNC-84) domain nuclear envelope (NE) proteins play a crucial role in meiotic chromosome movement and homologue pairing. However, although the vertebrate SUN domain protein SUN1 is involved in these processes, its partner has remained identified. Based on subcellular localization screening in mouse spermatocytes, we identified a novel germ cell–specific protein, KASH5, that localized exclusively at telomeres from the leptotene to diplotene stages in both spermatocytes and oocytes. KASH5 possesses hitherto unknown KASH-related sequences that directly interacted with SUN1 and mediated telomere localization. Thus, KASH5 is a mammalian meiosis-specific KASH domain protein. We show that meiotic chromosome movement depended on microtubules and that KASH5 interacted with the microtubule-associated dynein–dynactin complex. These results suggest that KASH5 connects the telomere-associated SUN1 protein to the cytoplasmic force–generating mechanism involved in meiotic chromosome movement. Our study strongly suggests that the meiotic homologue-pairing mechanism mediated by the SUN–KASH NE bridge is highly conserved among eukaryotes. The Rockefeller University Press 2012-07-23 /pmc/articles/PMC3410425/ /pubmed/22826121 http://dx.doi.org/10.1083/jcb.201204085 Text en © 2012 Morimoto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Morimoto, Akihiro
Shibuya, Hiroki
Zhu, Xiaoqiang
Kim, Jihye
Ishiguro, Kei-ichiro
Han, Min
Watanabe, Yoshinori
A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
title A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
title_full A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
title_fullStr A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
title_full_unstemmed A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
title_short A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
title_sort conserved kash domain protein associates with telomeres, sun1, and dynactin during mammalian meiosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410425/
https://www.ncbi.nlm.nih.gov/pubmed/22826121
http://dx.doi.org/10.1083/jcb.201204085
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