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AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication
AdpA, one of the most pleiotropic transcription regulators in bacteria, controls expression of several dozen genes during Streptomyces differentiation. Here, we report a novel function for the AdpA protein: inhibitor of chromosome replication at the initiation stage. AdpA specifically recognizes the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411110/ https://www.ncbi.nlm.nih.gov/pubmed/22870392 http://dx.doi.org/10.1098/rsob.120097 |
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author | Wolański, Marcin Jakimowicz, Dagmara Zakrzewska-Czerwińska, Jolanta |
author_facet | Wolański, Marcin Jakimowicz, Dagmara Zakrzewska-Czerwińska, Jolanta |
author_sort | Wolański, Marcin |
collection | PubMed |
description | AdpA, one of the most pleiotropic transcription regulators in bacteria, controls expression of several dozen genes during Streptomyces differentiation. Here, we report a novel function for the AdpA protein: inhibitor of chromosome replication at the initiation stage. AdpA specifically recognizes the 5′ region of the Streptomyces coelicolor replication origin (oriC). Our in vitro results show that binding of AdpA protein decreased access of initiator protein (DnaA) to the oriC region. We also found that mutation of AdpA-binding sequences increased the accessibility of oriC to DnaA, which led to more frequent replication and acceleration of Streptomyces differentiation (at the stage of aerial hyphae formation). Moreover, we also provide evidence that AdpA and DnaA proteins compete for oriC binding in an ATP-dependent manner, with low ATP levels causing preferential binding of AdpA, and high ATP levels causing dissociation of AdpA and association of DnaA. This would be consistent with a role for ATP levels in determining when aerial hyphae emerge. |
format | Online Article Text |
id | pubmed-3411110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-34111102012-08-06 AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication Wolański, Marcin Jakimowicz, Dagmara Zakrzewska-Czerwińska, Jolanta Open Biol Research AdpA, one of the most pleiotropic transcription regulators in bacteria, controls expression of several dozen genes during Streptomyces differentiation. Here, we report a novel function for the AdpA protein: inhibitor of chromosome replication at the initiation stage. AdpA specifically recognizes the 5′ region of the Streptomyces coelicolor replication origin (oriC). Our in vitro results show that binding of AdpA protein decreased access of initiator protein (DnaA) to the oriC region. We also found that mutation of AdpA-binding sequences increased the accessibility of oriC to DnaA, which led to more frequent replication and acceleration of Streptomyces differentiation (at the stage of aerial hyphae formation). Moreover, we also provide evidence that AdpA and DnaA proteins compete for oriC binding in an ATP-dependent manner, with low ATP levels causing preferential binding of AdpA, and high ATP levels causing dissociation of AdpA and association of DnaA. This would be consistent with a role for ATP levels in determining when aerial hyphae emerge. The Royal Society 2012-07 /pmc/articles/PMC3411110/ /pubmed/22870392 http://dx.doi.org/10.1098/rsob.120097 Text en http://creativecommons.org/licenses/by/3.0/ © 2012 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Wolański, Marcin Jakimowicz, Dagmara Zakrzewska-Czerwińska, Jolanta AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication |
title | AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication |
title_full | AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication |
title_fullStr | AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication |
title_full_unstemmed | AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication |
title_short | AdpA, key regulator for morphological differentiation regulates bacterial chromosome replication |
title_sort | adpa, key regulator for morphological differentiation regulates bacterial chromosome replication |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411110/ https://www.ncbi.nlm.nih.gov/pubmed/22870392 http://dx.doi.org/10.1098/rsob.120097 |
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