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Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction

INTRODUCTION: The expression of hundreds of genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV remodeling and cardiac performance after MI. However, the molecular basis is unknown. METHODS: MI was p...

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Autores principales: Chen, Yue-Feng, Pottala, James V., Weltman, Nathan Y., Ge, Xijin, Savinova, Olga V., Gerdes, A. Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411604/
https://www.ncbi.nlm.nih.gov/pubmed/22870193
http://dx.doi.org/10.1371/journal.pone.0040161
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author Chen, Yue-Feng
Pottala, James V.
Weltman, Nathan Y.
Ge, Xijin
Savinova, Olga V.
Gerdes, A. Martin
author_facet Chen, Yue-Feng
Pottala, James V.
Weltman, Nathan Y.
Ge, Xijin
Savinova, Olga V.
Gerdes, A. Martin
author_sort Chen, Yue-Feng
collection PubMed
description INTRODUCTION: The expression of hundreds of genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV remodeling and cardiac performance after MI. However, the molecular basis is unknown. METHODS: MI was produced by ligation of the left anterior descending coronary artery in female SD rats. Rats were divided into the following groups: (1) Sham MI, (2) MI, and (3) MI+T4 treatment (T4 pellet 3.3 mg, 60 days release, implanted subcutaneously immediately following MI). Four weeks after surgery, total RNA was isolated from LV non-infarcted areas for microarray analysis using the Illumina RatRef-12 Expression BeadChip Platform. RESULTS: Signals were detected in 13,188 genes (out of 22,523), of which the expression of 154 genes were decreased and the expression of 200 genes were increased in MI rats compared with Sham MI rats (false discovery rate (FDR) <0.05). Compared to MI rats, T4 treatment decreased expression of 27 genes and increased expression of 28 genes. In particular, 6 genes down-regulated by MI and 12 genes up-regulated by MI were reversed by T4. Most of the 55 genes altered by T4 treatment are in the category of molecular function under binding (24) and biological processes which includes immune system process (9), multi-organism process (5) and biological regulation (19) nonexclusively. CONCLUSIONS: These results suggest that altered expression of genes for molecular function and biological process may be involved in the beneficial effects of thyroid hormone treatment following MI in rats.
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spelling pubmed-34116042012-08-06 Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction Chen, Yue-Feng Pottala, James V. Weltman, Nathan Y. Ge, Xijin Savinova, Olga V. Gerdes, A. Martin PLoS One Research Article INTRODUCTION: The expression of hundreds of genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV remodeling and cardiac performance after MI. However, the molecular basis is unknown. METHODS: MI was produced by ligation of the left anterior descending coronary artery in female SD rats. Rats were divided into the following groups: (1) Sham MI, (2) MI, and (3) MI+T4 treatment (T4 pellet 3.3 mg, 60 days release, implanted subcutaneously immediately following MI). Four weeks after surgery, total RNA was isolated from LV non-infarcted areas for microarray analysis using the Illumina RatRef-12 Expression BeadChip Platform. RESULTS: Signals were detected in 13,188 genes (out of 22,523), of which the expression of 154 genes were decreased and the expression of 200 genes were increased in MI rats compared with Sham MI rats (false discovery rate (FDR) <0.05). Compared to MI rats, T4 treatment decreased expression of 27 genes and increased expression of 28 genes. In particular, 6 genes down-regulated by MI and 12 genes up-regulated by MI were reversed by T4. Most of the 55 genes altered by T4 treatment are in the category of molecular function under binding (24) and biological processes which includes immune system process (9), multi-organism process (5) and biological regulation (19) nonexclusively. CONCLUSIONS: These results suggest that altered expression of genes for molecular function and biological process may be involved in the beneficial effects of thyroid hormone treatment following MI in rats. Public Library of Science 2012-08-01 /pmc/articles/PMC3411604/ /pubmed/22870193 http://dx.doi.org/10.1371/journal.pone.0040161 Text en © 2012 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Yue-Feng
Pottala, James V.
Weltman, Nathan Y.
Ge, Xijin
Savinova, Olga V.
Gerdes, A. Martin
Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction
title Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction
title_full Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction
title_fullStr Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction
title_full_unstemmed Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction
title_short Regulation of Gene Expression with Thyroid Hormone in Rats with Myocardial Infarction
title_sort regulation of gene expression with thyroid hormone in rats with myocardial infarction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411604/
https://www.ncbi.nlm.nih.gov/pubmed/22870193
http://dx.doi.org/10.1371/journal.pone.0040161
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