Cargando…

Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil

Cardiac morphology and function assessment by magnetic resonance imaging is of increasing interest for a variety of mouse models in pre-clinical cardiac research, such as myocardial infarction models or myocardial injury/remodeling in genetically or pharmacologically induced hypertension. Signal-to-...

Descripción completa

Detalles Bibliográficos
Autores principales: Wagenhaus, Babette, Pohlmann, Andreas, Dieringer, Matthias Alexander, Els, Antje, Waiczies, Helmar, Waiczies, Sonia, Schulz-Menger, Jeanette, Niendorf, Thoralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411643/
https://www.ncbi.nlm.nih.gov/pubmed/22870323
http://dx.doi.org/10.1371/journal.pone.0042383
_version_ 1782239863099097088
author Wagenhaus, Babette
Pohlmann, Andreas
Dieringer, Matthias Alexander
Els, Antje
Waiczies, Helmar
Waiczies, Sonia
Schulz-Menger, Jeanette
Niendorf, Thoralf
author_facet Wagenhaus, Babette
Pohlmann, Andreas
Dieringer, Matthias Alexander
Els, Antje
Waiczies, Helmar
Waiczies, Sonia
Schulz-Menger, Jeanette
Niendorf, Thoralf
author_sort Wagenhaus, Babette
collection PubMed
description Cardiac morphology and function assessment by magnetic resonance imaging is of increasing interest for a variety of mouse models in pre-clinical cardiac research, such as myocardial infarction models or myocardial injury/remodeling in genetically or pharmacologically induced hypertension. Signal-to-noise ratio (SNR) constraints, however, limit image quality and blood myocardium delineation, which crucially depend on high spatial resolution. Significant gains in SNR with a cryogenically cooled RF probe have been shown for mouse brain MRI, yet the potential of applying cryogenic RF coils for cardiac MR (CMR) in mice is, as of yet, untapped. This study examines the feasibility and potential benefits of CMR in mice employing a 400 MHz cryogenic RF surface coil, compared with a conventional mouse heart coil array operating at room temperature. The cryogenic RF coil affords SNR gains of 3.0 to 5.0 versus the conventional approach and hence enables an enhanced spatial resolution. This markedly improved image quality – by better deliniation of myocardial borders and enhanced depiction of papillary muscles and trabeculae – and facilitated a more accurate cardiac chamber quantification, due to reduced intraobserver variability. In summary the use of a cryogenically cooled RF probe represents a valuable means of enhancing the capabilities of CMR of mice.
format Online
Article
Text
id pubmed-3411643
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34116432012-08-06 Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil Wagenhaus, Babette Pohlmann, Andreas Dieringer, Matthias Alexander Els, Antje Waiczies, Helmar Waiczies, Sonia Schulz-Menger, Jeanette Niendorf, Thoralf PLoS One Research Article Cardiac morphology and function assessment by magnetic resonance imaging is of increasing interest for a variety of mouse models in pre-clinical cardiac research, such as myocardial infarction models or myocardial injury/remodeling in genetically or pharmacologically induced hypertension. Signal-to-noise ratio (SNR) constraints, however, limit image quality and blood myocardium delineation, which crucially depend on high spatial resolution. Significant gains in SNR with a cryogenically cooled RF probe have been shown for mouse brain MRI, yet the potential of applying cryogenic RF coils for cardiac MR (CMR) in mice is, as of yet, untapped. This study examines the feasibility and potential benefits of CMR in mice employing a 400 MHz cryogenic RF surface coil, compared with a conventional mouse heart coil array operating at room temperature. The cryogenic RF coil affords SNR gains of 3.0 to 5.0 versus the conventional approach and hence enables an enhanced spatial resolution. This markedly improved image quality – by better deliniation of myocardial borders and enhanced depiction of papillary muscles and trabeculae – and facilitated a more accurate cardiac chamber quantification, due to reduced intraobserver variability. In summary the use of a cryogenically cooled RF probe represents a valuable means of enhancing the capabilities of CMR of mice. Public Library of Science 2012-08-01 /pmc/articles/PMC3411643/ /pubmed/22870323 http://dx.doi.org/10.1371/journal.pone.0042383 Text en © 2012 Wagenhaus et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wagenhaus, Babette
Pohlmann, Andreas
Dieringer, Matthias Alexander
Els, Antje
Waiczies, Helmar
Waiczies, Sonia
Schulz-Menger, Jeanette
Niendorf, Thoralf
Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil
title Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil
title_full Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil
title_fullStr Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil
title_full_unstemmed Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil
title_short Functional and Morphological Cardiac Magnetic Resonance Imaging of Mice Using a Cryogenic Quadrature Radiofrequency Coil
title_sort functional and morphological cardiac magnetic resonance imaging of mice using a cryogenic quadrature radiofrequency coil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411643/
https://www.ncbi.nlm.nih.gov/pubmed/22870323
http://dx.doi.org/10.1371/journal.pone.0042383
work_keys_str_mv AT wagenhausbabette functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT pohlmannandreas functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT dieringermatthiasalexander functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT elsantje functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT waiczieshelmar functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT waicziessonia functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT schulzmengerjeanette functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil
AT niendorfthoralf functionalandmorphologicalcardiacmagneticresonanceimagingofmiceusingacryogenicquadratureradiofrequencycoil