Optimized PCR Conditions and Increased shRNA Fold Representation Improve Reproducibility of Pooled shRNA Screens

RNAi screening using pooled shRNA libraries is a valuable tool for identifying genetic regulators of biological processes. However, for a successful pooled shRNA screen, it is imperative to thoroughly optimize experimental conditions to obtain reproducible data. Here we performed viability screens w...

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Detalles Bibliográficos
Autores principales: Strezoska, Žaklina, Licon, Abel, Haimes, Josh, Spayd, Katie Jansen, Patel, Kruti M., Sullivan, Kevin, Jastrzebski, Katarzyna, Simpson, Kaylene J., Leake, Devin, van Brabant Smith, Anja, Vermeulen, Annaleen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411659/
https://www.ncbi.nlm.nih.gov/pubmed/22870320
http://dx.doi.org/10.1371/journal.pone.0042341

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