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In Situ Optical Mapping of Voltage and Calcium in the Heart
Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological funct...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411684/ https://www.ncbi.nlm.nih.gov/pubmed/22876327 http://dx.doi.org/10.1371/journal.pone.0042562 |
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author | Lee, Peter Taghavi, Fouad Yan, Ping Ewart, Paul Ashley, Euan A. Loew, Leslie M. Kohl, Peter Bollensdorff, Christian Woods, Christopher E. |
author_facet | Lee, Peter Taghavi, Fouad Yan, Ping Ewart, Paul Ashley, Euan A. Loew, Leslie M. Kohl, Peter Bollensdorff, Christian Woods, Christopher E. |
author_sort | Lee, Peter |
collection | PubMed |
description | Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches. |
format | Online Article Text |
id | pubmed-3411684 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34116842012-08-08 In Situ Optical Mapping of Voltage and Calcium in the Heart Lee, Peter Taghavi, Fouad Yan, Ping Ewart, Paul Ashley, Euan A. Loew, Leslie M. Kohl, Peter Bollensdorff, Christian Woods, Christopher E. PLoS One Research Article Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches. Public Library of Science 2012-08-02 /pmc/articles/PMC3411684/ /pubmed/22876327 http://dx.doi.org/10.1371/journal.pone.0042562 Text en © 2012 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lee, Peter Taghavi, Fouad Yan, Ping Ewart, Paul Ashley, Euan A. Loew, Leslie M. Kohl, Peter Bollensdorff, Christian Woods, Christopher E. In Situ Optical Mapping of Voltage and Calcium in the Heart |
title |
In Situ Optical Mapping of Voltage and Calcium in the Heart |
title_full |
In Situ Optical Mapping of Voltage and Calcium in the Heart |
title_fullStr |
In Situ Optical Mapping of Voltage and Calcium in the Heart |
title_full_unstemmed |
In Situ Optical Mapping of Voltage and Calcium in the Heart |
title_short |
In Situ Optical Mapping of Voltage and Calcium in the Heart |
title_sort | in situ optical mapping of voltage and calcium in the heart |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411684/ https://www.ncbi.nlm.nih.gov/pubmed/22876327 http://dx.doi.org/10.1371/journal.pone.0042562 |
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