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Predicting Human Age with Bloodstains by sjTREC Quantification

The age-related decline of signal joint T-cell receptor rearrangement excision circles (sjTRECs) in human peripheral blood has been demonstrated in our previous study and other reports. Until now, only a few studies on sjTREC detection in bloodstain samples were reported, which were based on a small...

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Autores principales: Ou, Xue-ling, Gao, Jun, Wang, Huan, Wang, Hong-sheng, Lu, Hui-ling, Sun, Hong-yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411734/
https://www.ncbi.nlm.nih.gov/pubmed/22879970
http://dx.doi.org/10.1371/journal.pone.0042412
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author Ou, Xue-ling
Gao, Jun
Wang, Huan
Wang, Hong-sheng
Lu, Hui-ling
Sun, Hong-yu
author_facet Ou, Xue-ling
Gao, Jun
Wang, Huan
Wang, Hong-sheng
Lu, Hui-ling
Sun, Hong-yu
author_sort Ou, Xue-ling
collection PubMed
description The age-related decline of signal joint T-cell receptor rearrangement excision circles (sjTRECs) in human peripheral blood has been demonstrated in our previous study and other reports. Until now, only a few studies on sjTREC detection in bloodstain samples were reported, which were based on a small sample of subjects of a limited age range, although bloodstains are much more frequently encountered in forensic practice. In this present study, we adopted the sensitive Taqman real-time quantitative polymerase chain reaction (qPCR) method to perform sjTREC quantification in bloodstains from individuals ranging from 0–86 years old (n = 264). The results revealed that sjTREC contents in human bloodstains were declined in an age-dependent manner (r = −0.8712). The formula of age estimation was Age  = −7.1815Y−42.458±9.42 (Y dCt(TBP-sjTREC); 9.42 standard error). Furthermore, we tested for the influence of short- or long- storage time by analyzing fresh and stored bloodstains from the same individuals. Remarkably, no statistically significant difference in sjTREC contents was found between the fresh and old DNA samples over a 4-week of storage time. However, significant loss (0.16–1.93 dCt) in sjTREC contents was detected after 1.5 years of storage in 31 samples. Moreover, preliminary sjTREC quantification from up to 20-year-old bloodstains showed that though the sjTREC contents were detectable in all samples and highly correlated with donor age, a time-dependent decrease in the correlation coefficient r was found, suggesting the predicting accuracy of this described assay would be deteriorated in aged samples. Our findings show that sjTREC quantification might be also suitable for age prediction in bloodstains, and future researches into the time-dependent or other potential impacts on sjTREC quantification might allow further improvement of the predicting accuracy.
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spelling pubmed-34117342012-08-09 Predicting Human Age with Bloodstains by sjTREC Quantification Ou, Xue-ling Gao, Jun Wang, Huan Wang, Hong-sheng Lu, Hui-ling Sun, Hong-yu PLoS One Research Article The age-related decline of signal joint T-cell receptor rearrangement excision circles (sjTRECs) in human peripheral blood has been demonstrated in our previous study and other reports. Until now, only a few studies on sjTREC detection in bloodstain samples were reported, which were based on a small sample of subjects of a limited age range, although bloodstains are much more frequently encountered in forensic practice. In this present study, we adopted the sensitive Taqman real-time quantitative polymerase chain reaction (qPCR) method to perform sjTREC quantification in bloodstains from individuals ranging from 0–86 years old (n = 264). The results revealed that sjTREC contents in human bloodstains were declined in an age-dependent manner (r = −0.8712). The formula of age estimation was Age  = −7.1815Y−42.458±9.42 (Y dCt(TBP-sjTREC); 9.42 standard error). Furthermore, we tested for the influence of short- or long- storage time by analyzing fresh and stored bloodstains from the same individuals. Remarkably, no statistically significant difference in sjTREC contents was found between the fresh and old DNA samples over a 4-week of storage time. However, significant loss (0.16–1.93 dCt) in sjTREC contents was detected after 1.5 years of storage in 31 samples. Moreover, preliminary sjTREC quantification from up to 20-year-old bloodstains showed that though the sjTREC contents were detectable in all samples and highly correlated with donor age, a time-dependent decrease in the correlation coefficient r was found, suggesting the predicting accuracy of this described assay would be deteriorated in aged samples. Our findings show that sjTREC quantification might be also suitable for age prediction in bloodstains, and future researches into the time-dependent or other potential impacts on sjTREC quantification might allow further improvement of the predicting accuracy. Public Library of Science 2012-08-03 /pmc/articles/PMC3411734/ /pubmed/22879970 http://dx.doi.org/10.1371/journal.pone.0042412 Text en © 2012 Ou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ou, Xue-ling
Gao, Jun
Wang, Huan
Wang, Hong-sheng
Lu, Hui-ling
Sun, Hong-yu
Predicting Human Age with Bloodstains by sjTREC Quantification
title Predicting Human Age with Bloodstains by sjTREC Quantification
title_full Predicting Human Age with Bloodstains by sjTREC Quantification
title_fullStr Predicting Human Age with Bloodstains by sjTREC Quantification
title_full_unstemmed Predicting Human Age with Bloodstains by sjTREC Quantification
title_short Predicting Human Age with Bloodstains by sjTREC Quantification
title_sort predicting human age with bloodstains by sjtrec quantification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411734/
https://www.ncbi.nlm.nih.gov/pubmed/22879970
http://dx.doi.org/10.1371/journal.pone.0042412
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