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Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits
BACKGROUND: Hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels generate the pacemaking current, I(h), which regulates neuronal excitability, burst firing activity, rhythmogenesis, and synaptic integration. The physiological consequence of HCN activation depends on regulation of chan...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411840/ https://www.ncbi.nlm.nih.gov/pubmed/22880098 http://dx.doi.org/10.1371/journal.pone.0042756 |
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author | Ying, Shui-Wang Kanda, Vikram A. Hu, Zhaoyang Purtell, Kerry King, Elizabeth C. Abbott, Geoffrey W. Goldstein, Peter A. |
author_facet | Ying, Shui-Wang Kanda, Vikram A. Hu, Zhaoyang Purtell, Kerry King, Elizabeth C. Abbott, Geoffrey W. Goldstein, Peter A. |
author_sort | Ying, Shui-Wang |
collection | PubMed |
description | BACKGROUND: Hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels generate the pacemaking current, I(h), which regulates neuronal excitability, burst firing activity, rhythmogenesis, and synaptic integration. The physiological consequence of HCN activation depends on regulation of channel gating by endogenous modulators and stabilization of the channel complex formed by principal and ancillary subunits. KCNE2 is a voltage-gated potassium channel ancillary subunit that also regulates heterologously expressed HCN channels; whether KCNE2 regulates neuronal HCN channel function is unknown. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the effects of Kcne2 gene deletion on I(h) properties and excitability in ventrobasal (VB) and cortical layer 6 pyramidal neurons using brain slices prepared from Kcne2 (+/+) and Kcne2 (−/−) mice. Kcne2 deletion shifted the voltage-dependence of I(h) activation to more hyperpolarized potentials, slowed gating kinetics, and decreased I(h) density. Kcne2 deletion was associated with a reduction in whole-brain expression of both HCN1 and HCN2 (but not HCN4), although co-immunoprecipitation from whole-brain lysates failed to detect interaction of KCNE2 with HCN1 or 2. Kcne2 deletion also increased input resistance and temporal summation of subthreshold voltage responses; this increased intrinsic excitability enhanced burst firing in response to 4-aminopyridine. Burst duration increased in corticothalamic, but not thalamocortical, neurons, suggesting enhanced cortical excitatory input to the thalamus; such augmented excitability did not result from changes in glutamate release machinery since miniature EPSC frequency was unaltered in Kcne2 (−/−) neurons. CONCLUSIONS/SIGNIFICANCE: Loss of KCNE2 leads to downregulation of HCN channel function associated with increased excitability in neurons in the cortico-thalamo-cortical loop. Such findings further our understanding of the normal physiology of brain circuitry critically involved in cognition and have implications for our understanding of various disorders of consciousness. |
format | Online Article Text |
id | pubmed-3411840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34118402012-08-09 Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits Ying, Shui-Wang Kanda, Vikram A. Hu, Zhaoyang Purtell, Kerry King, Elizabeth C. Abbott, Geoffrey W. Goldstein, Peter A. PLoS One Research Article BACKGROUND: Hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels generate the pacemaking current, I(h), which regulates neuronal excitability, burst firing activity, rhythmogenesis, and synaptic integration. The physiological consequence of HCN activation depends on regulation of channel gating by endogenous modulators and stabilization of the channel complex formed by principal and ancillary subunits. KCNE2 is a voltage-gated potassium channel ancillary subunit that also regulates heterologously expressed HCN channels; whether KCNE2 regulates neuronal HCN channel function is unknown. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the effects of Kcne2 gene deletion on I(h) properties and excitability in ventrobasal (VB) and cortical layer 6 pyramidal neurons using brain slices prepared from Kcne2 (+/+) and Kcne2 (−/−) mice. Kcne2 deletion shifted the voltage-dependence of I(h) activation to more hyperpolarized potentials, slowed gating kinetics, and decreased I(h) density. Kcne2 deletion was associated with a reduction in whole-brain expression of both HCN1 and HCN2 (but not HCN4), although co-immunoprecipitation from whole-brain lysates failed to detect interaction of KCNE2 with HCN1 or 2. Kcne2 deletion also increased input resistance and temporal summation of subthreshold voltage responses; this increased intrinsic excitability enhanced burst firing in response to 4-aminopyridine. Burst duration increased in corticothalamic, but not thalamocortical, neurons, suggesting enhanced cortical excitatory input to the thalamus; such augmented excitability did not result from changes in glutamate release machinery since miniature EPSC frequency was unaltered in Kcne2 (−/−) neurons. CONCLUSIONS/SIGNIFICANCE: Loss of KCNE2 leads to downregulation of HCN channel function associated with increased excitability in neurons in the cortico-thalamo-cortical loop. Such findings further our understanding of the normal physiology of brain circuitry critically involved in cognition and have implications for our understanding of various disorders of consciousness. Public Library of Science 2012-08-03 /pmc/articles/PMC3411840/ /pubmed/22880098 http://dx.doi.org/10.1371/journal.pone.0042756 Text en © 2012 Ying et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ying, Shui-Wang Kanda, Vikram A. Hu, Zhaoyang Purtell, Kerry King, Elizabeth C. Abbott, Geoffrey W. Goldstein, Peter A. Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits |
title | Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits |
title_full | Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits |
title_fullStr | Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits |
title_full_unstemmed | Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits |
title_short | Targeted Deletion of Kcne2 Impairs HCN Channel Function in Mouse Thalamocortical Circuits |
title_sort | targeted deletion of kcne2 impairs hcn channel function in mouse thalamocortical circuits |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411840/ https://www.ncbi.nlm.nih.gov/pubmed/22880098 http://dx.doi.org/10.1371/journal.pone.0042756 |
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