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Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth

Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis) that were...

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Detalles Bibliográficos
Autores principales: Hamrahi, Victoria, Hamblin, Michael R., Jung, Walter, Benjamin, John B., Paul, Kasie W., Fischman, Alan J., Tompkins, Ronald G., Carter, Edward A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412104/
https://www.ncbi.nlm.nih.gov/pubmed/22899912
http://dx.doi.org/10.1155/2012/567140
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author Hamrahi, Victoria
Hamblin, Michael R.
Jung, Walter
Benjamin, John B.
Paul, Kasie W.
Fischman, Alan J.
Tompkins, Ronald G.
Carter, Edward A.
author_facet Hamrahi, Victoria
Hamblin, Michael R.
Jung, Walter
Benjamin, John B.
Paul, Kasie W.
Fischman, Alan J.
Tompkins, Ronald G.
Carter, Edward A.
author_sort Hamrahi, Victoria
collection PubMed
description Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis) that were genetically engineered to be bioluminescent, which allowed for noninvasive, sequential optical imaging of the extent and severity of the infection. The bioluminescent bacteria migrated from subcutaneous abscesses in the leg to distant burn wounds on the back depending on the severity of the burn injury, and this migration led to increased mortality of the mice. Treatment with ciprofloxacin, injected either in the leg with the bacterial infection or into the burn eschar, prevented this colonization of the wound and decreased mortality. The present data suggest that burn wounds can readily become colonized by infections distant from the wound itself.
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spelling pubmed-34121042012-08-16 Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth Hamrahi, Victoria Hamblin, Michael R. Jung, Walter Benjamin, John B. Paul, Kasie W. Fischman, Alan J. Tompkins, Ronald G. Carter, Edward A. Interdiscip Perspect Infect Dis Research Article Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis) that were genetically engineered to be bioluminescent, which allowed for noninvasive, sequential optical imaging of the extent and severity of the infection. The bioluminescent bacteria migrated from subcutaneous abscesses in the leg to distant burn wounds on the back depending on the severity of the burn injury, and this migration led to increased mortality of the mice. Treatment with ciprofloxacin, injected either in the leg with the bacterial infection or into the burn eschar, prevented this colonization of the wound and decreased mortality. The present data suggest that burn wounds can readily become colonized by infections distant from the wound itself. Hindawi Publishing Corporation 2012 2012-07-26 /pmc/articles/PMC3412104/ /pubmed/22899912 http://dx.doi.org/10.1155/2012/567140 Text en Copyright © 2012 Victoria Hamrahi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hamrahi, Victoria
Hamblin, Michael R.
Jung, Walter
Benjamin, John B.
Paul, Kasie W.
Fischman, Alan J.
Tompkins, Ronald G.
Carter, Edward A.
Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
title Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
title_full Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
title_fullStr Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
title_full_unstemmed Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
title_short Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
title_sort gram-negative bacterial infection in thigh abscess can migrate to distant burn depending on burn depth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412104/
https://www.ncbi.nlm.nih.gov/pubmed/22899912
http://dx.doi.org/10.1155/2012/567140
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