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Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth
Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis) that were...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412104/ https://www.ncbi.nlm.nih.gov/pubmed/22899912 http://dx.doi.org/10.1155/2012/567140 |
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author | Hamrahi, Victoria Hamblin, Michael R. Jung, Walter Benjamin, John B. Paul, Kasie W. Fischman, Alan J. Tompkins, Ronald G. Carter, Edward A. |
author_facet | Hamrahi, Victoria Hamblin, Michael R. Jung, Walter Benjamin, John B. Paul, Kasie W. Fischman, Alan J. Tompkins, Ronald G. Carter, Edward A. |
author_sort | Hamrahi, Victoria |
collection | PubMed |
description | Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis) that were genetically engineered to be bioluminescent, which allowed for noninvasive, sequential optical imaging of the extent and severity of the infection. The bioluminescent bacteria migrated from subcutaneous abscesses in the leg to distant burn wounds on the back depending on the severity of the burn injury, and this migration led to increased mortality of the mice. Treatment with ciprofloxacin, injected either in the leg with the bacterial infection or into the burn eschar, prevented this colonization of the wound and decreased mortality. The present data suggest that burn wounds can readily become colonized by infections distant from the wound itself. |
format | Online Article Text |
id | pubmed-3412104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34121042012-08-16 Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth Hamrahi, Victoria Hamblin, Michael R. Jung, Walter Benjamin, John B. Paul, Kasie W. Fischman, Alan J. Tompkins, Ronald G. Carter, Edward A. Interdiscip Perspect Infect Dis Research Article Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis) that were genetically engineered to be bioluminescent, which allowed for noninvasive, sequential optical imaging of the extent and severity of the infection. The bioluminescent bacteria migrated from subcutaneous abscesses in the leg to distant burn wounds on the back depending on the severity of the burn injury, and this migration led to increased mortality of the mice. Treatment with ciprofloxacin, injected either in the leg with the bacterial infection or into the burn eschar, prevented this colonization of the wound and decreased mortality. The present data suggest that burn wounds can readily become colonized by infections distant from the wound itself. Hindawi Publishing Corporation 2012 2012-07-26 /pmc/articles/PMC3412104/ /pubmed/22899912 http://dx.doi.org/10.1155/2012/567140 Text en Copyright © 2012 Victoria Hamrahi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hamrahi, Victoria Hamblin, Michael R. Jung, Walter Benjamin, John B. Paul, Kasie W. Fischman, Alan J. Tompkins, Ronald G. Carter, Edward A. Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth |
title | Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth |
title_full | Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth |
title_fullStr | Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth |
title_full_unstemmed | Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth |
title_short | Gram-Negative Bacterial Infection in Thigh Abscess Can Migrate to Distant Burn Depending on Burn Depth |
title_sort | gram-negative bacterial infection in thigh abscess can migrate to distant burn depending on burn depth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412104/ https://www.ncbi.nlm.nih.gov/pubmed/22899912 http://dx.doi.org/10.1155/2012/567140 |
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