Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline

As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed...

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Autores principales: Gabrielsen, Mads, Riboldi-Tunnicliffe, Alan, Ibáñez-Shimabukuro, Marina, Griffiths, Kate, Roe, Andrew J., Cooper, Alan, Smith, Brian O., Córsico, Betina, Kennedy, Malcolm W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2012
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412778/
https://www.ncbi.nlm.nih.gov/pubmed/22869127
http://dx.doi.org/10.1107/S1744309112026553
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author Gabrielsen, Mads
Riboldi-Tunnicliffe, Alan
Ibáñez-Shimabukuro, Marina
Griffiths, Kate
Roe, Andrew J.
Cooper, Alan
Smith, Brian O.
Córsico, Betina
Kennedy, Malcolm W.
author_facet Gabrielsen, Mads
Riboldi-Tunnicliffe, Alan
Ibáñez-Shimabukuro, Marina
Griffiths, Kate
Roe, Andrew J.
Cooper, Alan
Smith, Brian O.
Córsico, Betina
Kennedy, Malcolm W.
author_sort Gabrielsen, Mads
collection PubMed
description As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of ‘microdomains’. Interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm.
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spelling pubmed-34127782012-08-07 Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline Gabrielsen, Mads Riboldi-Tunnicliffe, Alan Ibáñez-Shimabukuro, Marina Griffiths, Kate Roe, Andrew J. Cooper, Alan Smith, Brian O. Córsico, Betina Kennedy, Malcolm W. Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of ‘microdomains’. Interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm. International Union of Crystallography 2012-07-31 /pmc/articles/PMC3412778/ /pubmed/22869127 http://dx.doi.org/10.1107/S1744309112026553 Text en © Gabrielsen et al. 2012 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Crystallization Communications
Gabrielsen, Mads
Riboldi-Tunnicliffe, Alan
Ibáñez-Shimabukuro, Marina
Griffiths, Kate
Roe, Andrew J.
Cooper, Alan
Smith, Brian O.
Córsico, Betina
Kennedy, Malcolm W.
Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline
title Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline
title_full Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline
title_fullStr Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline
title_full_unstemmed Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline
title_short Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline
title_sort useable diffraction data from a multiple microdomain-containing crystal of ascaris suum as-p18 fatty-acid-binding protein using a microfocus beamline
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412778/
https://www.ncbi.nlm.nih.gov/pubmed/22869127
http://dx.doi.org/10.1107/S1744309112026553
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