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MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells

BACKGROUND: Oxidative injury to retinal pigment epithelium (RPE) and retinal photoreceptors has been linked to a number of retinal diseases, including age-related macular degeneration (AMD). Reactive oxygen species (ROS)-mediated gene expression has been extensively studied at transcriptional levels...

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Autores principales: Haque, Rashidul, Chun, Eugene, Howell, Jennifer C., Sengupta, Trisha, Chen, Dan, Kim, Hana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412823/
https://www.ncbi.nlm.nih.gov/pubmed/22880027
http://dx.doi.org/10.1371/journal.pone.0042542
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author Haque, Rashidul
Chun, Eugene
Howell, Jennifer C.
Sengupta, Trisha
Chen, Dan
Kim, Hana
author_facet Haque, Rashidul
Chun, Eugene
Howell, Jennifer C.
Sengupta, Trisha
Chen, Dan
Kim, Hana
author_sort Haque, Rashidul
collection PubMed
description BACKGROUND: Oxidative injury to retinal pigment epithelium (RPE) and retinal photoreceptors has been linked to a number of retinal diseases, including age-related macular degeneration (AMD). Reactive oxygen species (ROS)-mediated gene expression has been extensively studied at transcriptional levels. Also, the post-transcriptional control of gene expression at the level of translational regulation has been recently reported. However, the microRNA (miRNA/miR)-mediated post-transcriptional regulation in human RPE cells has not been thoroughly looked at. Increasing evidence points to a potential role of miRNAs in diverse physiological processes. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrated for the first time in a human retinal pigment epithelial cell line (ARPE-19) that the post-transcriptional control of gene expression via miRNA modulation regulates human catalase, an important and potent component of cell's antioxidant defensive network, which detoxifies hydrogen peroxide (H(2)O(2)) radicals. Exposure to several stress-inducing agents including H(2)O(2) has been reported to alter miRNA expression profile. Here, we demonstrated that a sublethal dose of H(2)O(2) (200 µM) up-regulated the expression of miR-30b, a member of the miR-30 family, which inhibited the expression of endogenous catalase both at the transcript and protein levels. However, antisense (antagomirs) of miR-30b was not only found to suppress the miR-30b mimics-mediated inhibitions, but also to dramatically increase the expression of catalase even under an oxidant environment. CONCLUSIONS/SIGNIFICANCE: We propose that a microRNA antisense approach could enhance cytoprotective mechanisms against oxidative stress by increasing the antioxidant defense system.
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spelling pubmed-34128232012-08-09 MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells Haque, Rashidul Chun, Eugene Howell, Jennifer C. Sengupta, Trisha Chen, Dan Kim, Hana PLoS One Research Article BACKGROUND: Oxidative injury to retinal pigment epithelium (RPE) and retinal photoreceptors has been linked to a number of retinal diseases, including age-related macular degeneration (AMD). Reactive oxygen species (ROS)-mediated gene expression has been extensively studied at transcriptional levels. Also, the post-transcriptional control of gene expression at the level of translational regulation has been recently reported. However, the microRNA (miRNA/miR)-mediated post-transcriptional regulation in human RPE cells has not been thoroughly looked at. Increasing evidence points to a potential role of miRNAs in diverse physiological processes. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrated for the first time in a human retinal pigment epithelial cell line (ARPE-19) that the post-transcriptional control of gene expression via miRNA modulation regulates human catalase, an important and potent component of cell's antioxidant defensive network, which detoxifies hydrogen peroxide (H(2)O(2)) radicals. Exposure to several stress-inducing agents including H(2)O(2) has been reported to alter miRNA expression profile. Here, we demonstrated that a sublethal dose of H(2)O(2) (200 µM) up-regulated the expression of miR-30b, a member of the miR-30 family, which inhibited the expression of endogenous catalase both at the transcript and protein levels. However, antisense (antagomirs) of miR-30b was not only found to suppress the miR-30b mimics-mediated inhibitions, but also to dramatically increase the expression of catalase even under an oxidant environment. CONCLUSIONS/SIGNIFICANCE: We propose that a microRNA antisense approach could enhance cytoprotective mechanisms against oxidative stress by increasing the antioxidant defense system. Public Library of Science 2012-08-06 /pmc/articles/PMC3412823/ /pubmed/22880027 http://dx.doi.org/10.1371/journal.pone.0042542 Text en © 2012 Haque et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Haque, Rashidul
Chun, Eugene
Howell, Jennifer C.
Sengupta, Trisha
Chen, Dan
Kim, Hana
MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells
title MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells
title_full MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells
title_fullStr MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells
title_full_unstemmed MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells
title_short MicroRNA-30b-Mediated Regulation of Catalase Expression in Human ARPE-19 Cells
title_sort microrna-30b-mediated regulation of catalase expression in human arpe-19 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412823/
https://www.ncbi.nlm.nih.gov/pubmed/22880027
http://dx.doi.org/10.1371/journal.pone.0042542
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