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Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex
Homologous recombination (HR) represents a major error-free pathway to eliminate pre-carcinogenic chromosomal lesions. The DNA strand invasion reaction in HR is mediated by a helical filament of the Rad51 recombinase assembled on single-stranded DNA that is derived from the nucleolytic processing of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413116/ https://www.ncbi.nlm.nih.gov/pubmed/22492707 http://dx.doi.org/10.1093/nar/gks305 |
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author | Tsai, Shang-Pu Su, Guan-Chin Lin, Sheng-Wei Chung, Chan-I. Xue, Xiaoyu Dunlop, Myun Hwa Akamatsu, Yufuko Jasin, Maria Sung, Patrick Chi, Peter |
author_facet | Tsai, Shang-Pu Su, Guan-Chin Lin, Sheng-Wei Chung, Chan-I. Xue, Xiaoyu Dunlop, Myun Hwa Akamatsu, Yufuko Jasin, Maria Sung, Patrick Chi, Peter |
author_sort | Tsai, Shang-Pu |
collection | PubMed |
description | Homologous recombination (HR) represents a major error-free pathway to eliminate pre-carcinogenic chromosomal lesions. The DNA strand invasion reaction in HR is mediated by a helical filament of the Rad51 recombinase assembled on single-stranded DNA that is derived from the nucleolytic processing of the primary lesion. Recent studies have found that the human and mouse Swi5 and Sfr1 proteins form a complex that influences Rad51-mediated HR in cells. Here, we provide biophysical evidence that the mouse Swi5–Sfr1 complex has a 1:1 stoichiometry. Importantly, the Swi5–Sfr1 complex, but neither Swi5 nor Sfr1 alone, physically interacts with Rad51 and stimulates Rad51-mediated homologous DNA pairing. This stimulatory effect stems from the stabilization of the Rad51–ssDNA presynaptic filament. Moreover, we provide evidence that the RSfp (rodent Sfr1 proline rich) motif in Sfr1 serves as a negative regulatory element. These results thus reveal an evolutionarily conserved function in the Swi5–Sfr1 complex and furnish valuable information as to the regulatory role of the RSfp motif that isspecific to themammalianSfr1 orthologs. |
format | Online Article Text |
id | pubmed-3413116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34131162012-08-07 Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex Tsai, Shang-Pu Su, Guan-Chin Lin, Sheng-Wei Chung, Chan-I. Xue, Xiaoyu Dunlop, Myun Hwa Akamatsu, Yufuko Jasin, Maria Sung, Patrick Chi, Peter Nucleic Acids Res Genome Integrity, Repair and Replication Homologous recombination (HR) represents a major error-free pathway to eliminate pre-carcinogenic chromosomal lesions. The DNA strand invasion reaction in HR is mediated by a helical filament of the Rad51 recombinase assembled on single-stranded DNA that is derived from the nucleolytic processing of the primary lesion. Recent studies have found that the human and mouse Swi5 and Sfr1 proteins form a complex that influences Rad51-mediated HR in cells. Here, we provide biophysical evidence that the mouse Swi5–Sfr1 complex has a 1:1 stoichiometry. Importantly, the Swi5–Sfr1 complex, but neither Swi5 nor Sfr1 alone, physically interacts with Rad51 and stimulates Rad51-mediated homologous DNA pairing. This stimulatory effect stems from the stabilization of the Rad51–ssDNA presynaptic filament. Moreover, we provide evidence that the RSfp (rodent Sfr1 proline rich) motif in Sfr1 serves as a negative regulatory element. These results thus reveal an evolutionarily conserved function in the Swi5–Sfr1 complex and furnish valuable information as to the regulatory role of the RSfp motif that isspecific to themammalianSfr1 orthologs. Oxford University Press 2012-08 2012-04-09 /pmc/articles/PMC3413116/ /pubmed/22492707 http://dx.doi.org/10.1093/nar/gks305 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genome Integrity, Repair and Replication Tsai, Shang-Pu Su, Guan-Chin Lin, Sheng-Wei Chung, Chan-I. Xue, Xiaoyu Dunlop, Myun Hwa Akamatsu, Yufuko Jasin, Maria Sung, Patrick Chi, Peter Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex |
title | Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex |
title_full | Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex |
title_fullStr | Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex |
title_full_unstemmed | Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex |
title_short | Rad51 presynaptic filament stabilization function of the mouse Swi5–Sfr1 heterodimeric complex |
title_sort | rad51 presynaptic filament stabilization function of the mouse swi5–sfr1 heterodimeric complex |
topic | Genome Integrity, Repair and Replication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413116/ https://www.ncbi.nlm.nih.gov/pubmed/22492707 http://dx.doi.org/10.1093/nar/gks305 |
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