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Postnatal development- and age-related changes in DNA-methylation patterns in the human genome
Alterations in DNA methylation have been reported to occur during development and aging; however, much remains to be learned regarding post-natal and age-associated epigenome dynamics, and few if any investigations have compared human methylome patterns on a whole genome basis in cells from newborns...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413121/ https://www.ncbi.nlm.nih.gov/pubmed/22495928 http://dx.doi.org/10.1093/nar/gks312 |
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author | Salpea, Paraskevi Russanova, Valya R. Hirai, Tazuko H. Sourlingas, Thomae G. Sekeri-Pataryas, Kalliope E. Romero, Roberto Epstein, Jonathan Howard, Bruce H. |
author_facet | Salpea, Paraskevi Russanova, Valya R. Hirai, Tazuko H. Sourlingas, Thomae G. Sekeri-Pataryas, Kalliope E. Romero, Roberto Epstein, Jonathan Howard, Bruce H. |
author_sort | Salpea, Paraskevi |
collection | PubMed |
description | Alterations in DNA methylation have been reported to occur during development and aging; however, much remains to be learned regarding post-natal and age-associated epigenome dynamics, and few if any investigations have compared human methylome patterns on a whole genome basis in cells from newborns and adults. The aim of this study was to reveal genomic regions with distinct structure and sequence characteristics that render them subject to dynamic post-natal developmental remodeling or age-related dysregulation of epigenome structure. DNA samples derived from peripheral blood monocytes and in vitro differentiated dendritic cells were analyzed by methylated DNA Immunoprecipitation (MeDIP) or, for selected loci, bisulfite modification, followed by next generation sequencing. Regions of interest that emerged from the analysis included tandem or interspersed-tandem gene sequence repeats (PCDHG, FAM90A, HRNR, ECEL1P2), and genes with strong homology to other family members elsewhere in the genome (FZD1, FZD7 and FGF17). Our results raise the possibility that selected gene sequences with highly homologous copies may serve to facilitate, perhaps even provide a clock-like function for, developmental and age-related epigenome remodeling. If so, this would represent a fundamental feature of genome architecture in higher eukaryotic organisms. |
format | Online Article Text |
id | pubmed-3413121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34131212012-08-07 Postnatal development- and age-related changes in DNA-methylation patterns in the human genome Salpea, Paraskevi Russanova, Valya R. Hirai, Tazuko H. Sourlingas, Thomae G. Sekeri-Pataryas, Kalliope E. Romero, Roberto Epstein, Jonathan Howard, Bruce H. Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics Alterations in DNA methylation have been reported to occur during development and aging; however, much remains to be learned regarding post-natal and age-associated epigenome dynamics, and few if any investigations have compared human methylome patterns on a whole genome basis in cells from newborns and adults. The aim of this study was to reveal genomic regions with distinct structure and sequence characteristics that render them subject to dynamic post-natal developmental remodeling or age-related dysregulation of epigenome structure. DNA samples derived from peripheral blood monocytes and in vitro differentiated dendritic cells were analyzed by methylated DNA Immunoprecipitation (MeDIP) or, for selected loci, bisulfite modification, followed by next generation sequencing. Regions of interest that emerged from the analysis included tandem or interspersed-tandem gene sequence repeats (PCDHG, FAM90A, HRNR, ECEL1P2), and genes with strong homology to other family members elsewhere in the genome (FZD1, FZD7 and FGF17). Our results raise the possibility that selected gene sequences with highly homologous copies may serve to facilitate, perhaps even provide a clock-like function for, developmental and age-related epigenome remodeling. If so, this would represent a fundamental feature of genome architecture in higher eukaryotic organisms. Oxford University Press 2012-08 2012-04-11 /pmc/articles/PMC3413121/ /pubmed/22495928 http://dx.doi.org/10.1093/nar/gks312 Text en Published by Oxford University Press 2012. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Salpea, Paraskevi Russanova, Valya R. Hirai, Tazuko H. Sourlingas, Thomae G. Sekeri-Pataryas, Kalliope E. Romero, Roberto Epstein, Jonathan Howard, Bruce H. Postnatal development- and age-related changes in DNA-methylation patterns in the human genome |
title | Postnatal development- and age-related changes in DNA-methylation patterns in the human genome |
title_full | Postnatal development- and age-related changes in DNA-methylation patterns in the human genome |
title_fullStr | Postnatal development- and age-related changes in DNA-methylation patterns in the human genome |
title_full_unstemmed | Postnatal development- and age-related changes in DNA-methylation patterns in the human genome |
title_short | Postnatal development- and age-related changes in DNA-methylation patterns in the human genome |
title_sort | postnatal development- and age-related changes in dna-methylation patterns in the human genome |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413121/ https://www.ncbi.nlm.nih.gov/pubmed/22495928 http://dx.doi.org/10.1093/nar/gks312 |
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