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Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells

PURPOSE: Posterior capsular opacification (PCO) is caused mainly by the epithelial–mesenchymal transition (EMT), proliferation, and migration of human lens epithelial (HLE) cells. wingless (Wnt) signaling has been implicated in the fibrotic process by inducing EMT and increasing the proliferation of...

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Autores principales: Bao, Xiu-li, Song, Hui, Chen, Zhuo, Tang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413413/
https://www.ncbi.nlm.nih.gov/pubmed/22876125
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author Bao, Xiu-li
Song, Hui
Chen, Zhuo
Tang, Xin
author_facet Bao, Xiu-li
Song, Hui
Chen, Zhuo
Tang, Xin
author_sort Bao, Xiu-li
collection PubMed
description PURPOSE: Posterior capsular opacification (PCO) is caused mainly by the epithelial–mesenchymal transition (EMT), proliferation, and migration of human lens epithelial (HLE) cells. wingless (Wnt) signaling has been implicated in the fibrotic process by inducing EMT and increasing the proliferation of epithelial cells. This study investigated the role of Wnt3a in PCO formation. METHODS: Wnt3a was overexpressed in the HLE B-3 cell line by transfected Wnt3a-pcDNA3 plasmid. The expressions of Wnt/β-catenin signaling component proteins, including β-catenin, E-cadherin, fibronectin, c-Myc, and cyclin D1, were detected by western blot analysis and immunocytofluorescence to confirm the efficiency of transfection efficiency and analyze the effects of overexpression. HLE migration ability was evaluated by transwell migration and wound healing assays, whereas HLE proliferation was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl) −2,5-diphenyltetrazolium bromide] assay and flow cytometry. RESULTS: Overexpression of Wnt3a resulted in upregulated expression of β-catenin, c-Myc, and cyclin D1. Expression of the lens epithelial marker E-cadherin was down-regulated in Wnt3a-overexpressing HLE B-3 cells, whereas that of the mesenchymal marker fibronectin was upregulated. In addition, the morphology of HLE B-3 cells changed from the classic spindle shape to an irregular form. Overexpression of Wnt3a could enhance the ability of migration as determined by transwell migration and wound healing assays as well as promoted the proliferation of HLE B-3 cells by MTT assay and flow cytometry analysis. CONCLUSIONS: Wnt3a can induce EMT, migration, and proliferation of HLE cells and may be a valuable therapeutic target for the prevention and treatment of PCO.
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spelling pubmed-34134132012-08-08 Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells Bao, Xiu-li Song, Hui Chen, Zhuo Tang, Xin Mol Vis Research Article PURPOSE: Posterior capsular opacification (PCO) is caused mainly by the epithelial–mesenchymal transition (EMT), proliferation, and migration of human lens epithelial (HLE) cells. wingless (Wnt) signaling has been implicated in the fibrotic process by inducing EMT and increasing the proliferation of epithelial cells. This study investigated the role of Wnt3a in PCO formation. METHODS: Wnt3a was overexpressed in the HLE B-3 cell line by transfected Wnt3a-pcDNA3 plasmid. The expressions of Wnt/β-catenin signaling component proteins, including β-catenin, E-cadherin, fibronectin, c-Myc, and cyclin D1, were detected by western blot analysis and immunocytofluorescence to confirm the efficiency of transfection efficiency and analyze the effects of overexpression. HLE migration ability was evaluated by transwell migration and wound healing assays, whereas HLE proliferation was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl) −2,5-diphenyltetrazolium bromide] assay and flow cytometry. RESULTS: Overexpression of Wnt3a resulted in upregulated expression of β-catenin, c-Myc, and cyclin D1. Expression of the lens epithelial marker E-cadherin was down-regulated in Wnt3a-overexpressing HLE B-3 cells, whereas that of the mesenchymal marker fibronectin was upregulated. In addition, the morphology of HLE B-3 cells changed from the classic spindle shape to an irregular form. Overexpression of Wnt3a could enhance the ability of migration as determined by transwell migration and wound healing assays as well as promoted the proliferation of HLE B-3 cells by MTT assay and flow cytometry analysis. CONCLUSIONS: Wnt3a can induce EMT, migration, and proliferation of HLE cells and may be a valuable therapeutic target for the prevention and treatment of PCO. Molecular Vision 2012-07-18 /pmc/articles/PMC3413413/ /pubmed/22876125 Text en Copyright © 2012 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bao, Xiu-li
Song, Hui
Chen, Zhuo
Tang, Xin
Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
title Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
title_full Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
title_fullStr Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
title_full_unstemmed Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
title_short Wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
title_sort wnt3a promotes epithelial–mesenchymal transition, migration, and proliferation of lens epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413413/
https://www.ncbi.nlm.nih.gov/pubmed/22876125
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