Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2

PURPOSE: Extracellular matrix (ECM) deposits lead to elevated resistance of aqueous humor outflow which play an important role in the development of primary open angle glaucoma (POAG). The TGF-β2 (transforming growth factor β)/Smad (signaling mathers against decapentaplegic) pathway is known to regu...

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Autores principales: Su, Ying, Yang, Chen-Yuan C., Li, Zhongrui, Xu, Feng, Zhang, Lei, Wang, Feng, Zhao, Shiguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413437/
https://www.ncbi.nlm.nih.gov/pubmed/22876112
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author Su, Ying
Yang, Chen-Yuan C.
Li, Zhongrui
Xu, Feng
Zhang, Lei
Wang, Feng
Zhao, Shiguang
author_facet Su, Ying
Yang, Chen-Yuan C.
Li, Zhongrui
Xu, Feng
Zhang, Lei
Wang, Feng
Zhao, Shiguang
author_sort Su, Ying
collection PubMed
description PURPOSE: Extracellular matrix (ECM) deposits lead to elevated resistance of aqueous humor outflow which play an important role in the development of primary open angle glaucoma (POAG). The TGF-β2 (transforming growth factor β)/Smad (signaling mathers against decapentaplegic) pathway is known to regulate the ECM deposits. In this study, we determined the effect of Smad7 siRNA transfection in inhibiting the expression of ECM components. METHODS: Plasmid containing Smad7 siRNA was used to transfect cultured human trabecular meshwork cells (HTM). Protein expression of Smad7, fibronectin, and laminin was determined using western blot. RESULTS: Downregulation of Smad7 interrupts the effects of TGF-β2 on the expression of several ECM components. Smad7 siRNA can partially decrease the expression of Smad7, fibronectin, and laminin. CONCLUSIONS: Smad7 plays an important role in regulating the ECM protein in the aqueous outflow pathway.
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spelling pubmed-34134372012-08-08 Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2 Su, Ying Yang, Chen-Yuan C. Li, Zhongrui Xu, Feng Zhang, Lei Wang, Feng Zhao, Shiguang Mol Vis Research Article PURPOSE: Extracellular matrix (ECM) deposits lead to elevated resistance of aqueous humor outflow which play an important role in the development of primary open angle glaucoma (POAG). The TGF-β2 (transforming growth factor β)/Smad (signaling mathers against decapentaplegic) pathway is known to regulate the ECM deposits. In this study, we determined the effect of Smad7 siRNA transfection in inhibiting the expression of ECM components. METHODS: Plasmid containing Smad7 siRNA was used to transfect cultured human trabecular meshwork cells (HTM). Protein expression of Smad7, fibronectin, and laminin was determined using western blot. RESULTS: Downregulation of Smad7 interrupts the effects of TGF-β2 on the expression of several ECM components. Smad7 siRNA can partially decrease the expression of Smad7, fibronectin, and laminin. CONCLUSIONS: Smad7 plays an important role in regulating the ECM protein in the aqueous outflow pathway. Molecular Vision 2012-07-11 /pmc/articles/PMC3413437/ /pubmed/22876112 Text en Copyright © 2012 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Su, Ying
Yang, Chen-Yuan C.
Li, Zhongrui
Xu, Feng
Zhang, Lei
Wang, Feng
Zhao, Shiguang
Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2
title Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2
title_full Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2
title_fullStr Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2
title_full_unstemmed Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2
title_short Smad7 siRNA inhibit expression of extracellular matrix in trabecular meshwork cells treated with TGF-β2
title_sort smad7 sirna inhibit expression of extracellular matrix in trabecular meshwork cells treated with tgf-β2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413437/
https://www.ncbi.nlm.nih.gov/pubmed/22876112
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