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The complement system of the goat: Haemolytic assays and isolation of major proteins

BACKGROUND: The aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins. RESULTS: The commonly used sheep erythrocyte sensitized with rabbit antibodies were...

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Autores principales: Moreno-Indias, Isabel, Dodds, Alister W, Argüello, Anastasio, Castro, Noemi, Sim, Robert B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413586/
https://www.ncbi.nlm.nih.gov/pubmed/22734447
http://dx.doi.org/10.1186/1746-6148-8-91
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author Moreno-Indias, Isabel
Dodds, Alister W
Argüello, Anastasio
Castro, Noemi
Sim, Robert B
author_facet Moreno-Indias, Isabel
Dodds, Alister W
Argüello, Anastasio
Castro, Noemi
Sim, Robert B
author_sort Moreno-Indias, Isabel
collection PubMed
description BACKGROUND: The aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins. RESULTS: The commonly used sheep erythrocyte sensitized with rabbit antibodies were not sensitive to lysis by goat serum, but the combination of human red blood cells (RBC) plus rabbit antibodies was the best option found for goat complement assay. A buffer based on HEPES instead of the classical veronal (barbitone) was developed. Three proteins were isolated: factor H, C1q and C3 and these were compared with the corresponding human proteins. A novel affinity chromatography technique was developed for isolation of factor H. CONCLUSIONS: Human RBC plus rabbit antibodies were a suitable option for haemolytic assays. The isolated proteins are similar to the human counterparts.
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spelling pubmed-34135862012-08-08 The complement system of the goat: Haemolytic assays and isolation of major proteins Moreno-Indias, Isabel Dodds, Alister W Argüello, Anastasio Castro, Noemi Sim, Robert B BMC Vet Res Methodology Article BACKGROUND: The aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins. RESULTS: The commonly used sheep erythrocyte sensitized with rabbit antibodies were not sensitive to lysis by goat serum, but the combination of human red blood cells (RBC) plus rabbit antibodies was the best option found for goat complement assay. A buffer based on HEPES instead of the classical veronal (barbitone) was developed. Three proteins were isolated: factor H, C1q and C3 and these were compared with the corresponding human proteins. A novel affinity chromatography technique was developed for isolation of factor H. CONCLUSIONS: Human RBC plus rabbit antibodies were a suitable option for haemolytic assays. The isolated proteins are similar to the human counterparts. BioMed Central 2012-06-26 /pmc/articles/PMC3413586/ /pubmed/22734447 http://dx.doi.org/10.1186/1746-6148-8-91 Text en Copyright ©2012 Moreno-Indias et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Moreno-Indias, Isabel
Dodds, Alister W
Argüello, Anastasio
Castro, Noemi
Sim, Robert B
The complement system of the goat: Haemolytic assays and isolation of major proteins
title The complement system of the goat: Haemolytic assays and isolation of major proteins
title_full The complement system of the goat: Haemolytic assays and isolation of major proteins
title_fullStr The complement system of the goat: Haemolytic assays and isolation of major proteins
title_full_unstemmed The complement system of the goat: Haemolytic assays and isolation of major proteins
title_short The complement system of the goat: Haemolytic assays and isolation of major proteins
title_sort complement system of the goat: haemolytic assays and isolation of major proteins
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413586/
https://www.ncbi.nlm.nih.gov/pubmed/22734447
http://dx.doi.org/10.1186/1746-6148-8-91
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