Hepatitis E Virus Genotype 3 in Wild Rats, United States

The role of rodents in the epidemiology of zoonotic hepatitis E virus (HEV) infection has been a subject of considerable debate. Seroprevalence studies suggest widespread HEV infection in commensal Rattus spp. rats, but experimental transmission has been largely unsuccessful and recovery of zoonotic...

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Autores principales: Lack, Justin B., Volk, Kylie, Van Den Bussche, Ronald A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414038/
https://www.ncbi.nlm.nih.gov/pubmed/22840202
http://dx.doi.org/10.3201/eid1808.120070
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author Lack, Justin B.
Volk, Kylie
Van Den Bussche, Ronald A.
author_facet Lack, Justin B.
Volk, Kylie
Van Den Bussche, Ronald A.
author_sort Lack, Justin B.
collection PubMed
description The role of rodents in the epidemiology of zoonotic hepatitis E virus (HEV) infection has been a subject of considerable debate. Seroprevalence studies suggest widespread HEV infection in commensal Rattus spp. rats, but experimental transmission has been largely unsuccessful and recovery of zoonotic genotype 3 HEV RNA from wild Rattus spp. rats has never been confirmed. We surveyed R. rattus and R. norvegicus rats from across the United States and several international populations by using a hemi-nested reverse transcription PCR approach. We isolated HEV RNA in liver tissues from 35 of 446 rats examined. All but 1 of these isolates was relegated to the zoonotic HEV genotype 3, and the remaining sequence represented the recently discovered rat genotype from the United States and Germany. HEV-positive rats were detected in urban and remote localities. Genetic analyses suggest all HEV genotype 3 isolates obtained from wild Rattus spp. rats were closely related.
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spelling pubmed-34140382012-08-21 Hepatitis E Virus Genotype 3 in Wild Rats, United States Lack, Justin B. Volk, Kylie Van Den Bussche, Ronald A. Emerg Infect Dis Research The role of rodents in the epidemiology of zoonotic hepatitis E virus (HEV) infection has been a subject of considerable debate. Seroprevalence studies suggest widespread HEV infection in commensal Rattus spp. rats, but experimental transmission has been largely unsuccessful and recovery of zoonotic genotype 3 HEV RNA from wild Rattus spp. rats has never been confirmed. We surveyed R. rattus and R. norvegicus rats from across the United States and several international populations by using a hemi-nested reverse transcription PCR approach. We isolated HEV RNA in liver tissues from 35 of 446 rats examined. All but 1 of these isolates was relegated to the zoonotic HEV genotype 3, and the remaining sequence represented the recently discovered rat genotype from the United States and Germany. HEV-positive rats were detected in urban and remote localities. Genetic analyses suggest all HEV genotype 3 isolates obtained from wild Rattus spp. rats were closely related. Centers for Disease Control and Prevention 2012-08 /pmc/articles/PMC3414038/ /pubmed/22840202 http://dx.doi.org/10.3201/eid1808.120070 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Lack, Justin B.
Volk, Kylie
Van Den Bussche, Ronald A.
Hepatitis E Virus Genotype 3 in Wild Rats, United States
title Hepatitis E Virus Genotype 3 in Wild Rats, United States
title_full Hepatitis E Virus Genotype 3 in Wild Rats, United States
title_fullStr Hepatitis E Virus Genotype 3 in Wild Rats, United States
title_full_unstemmed Hepatitis E Virus Genotype 3 in Wild Rats, United States
title_short Hepatitis E Virus Genotype 3 in Wild Rats, United States
title_sort hepatitis e virus genotype 3 in wild rats, united states
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414038/
https://www.ncbi.nlm.nih.gov/pubmed/22840202
http://dx.doi.org/10.3201/eid1808.120070
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