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Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics

Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However,...

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Detalles Bibliográficos
Autores principales: Wu, Ting, Wang, Yejun, Guo, Dianjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414485/
https://www.ncbi.nlm.nih.gov/pubmed/22905110
http://dx.doi.org/10.1371/journal.pone.0041822
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author Wu, Ting
Wang, Yejun
Guo, Dianjing
author_facet Wu, Ting
Wang, Yejun
Guo, Dianjing
author_sort Wu, Ting
collection PubMed
description Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%∼1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism. 2D-electrophoresis was employed to compare the protein profiles of GSTs and leaves. More than 700 spots were resolved for GSTs, of which ∼93 non-redundant proteins were confidently identified by searching NCBI and Artemisia EST databases. Over 70% of these proteins were highly expressed in GTSs. Functional classification of these GSTs enriched proteins revealed that many of them participate in major plant metabolic processes such as electron transport, transcription and translation.
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spelling pubmed-34144852012-08-19 Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics Wu, Ting Wang, Yejun Guo, Dianjing PLoS One Research Article Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%∼1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism. 2D-electrophoresis was employed to compare the protein profiles of GSTs and leaves. More than 700 spots were resolved for GSTs, of which ∼93 non-redundant proteins were confidently identified by searching NCBI and Artemisia EST databases. Over 70% of these proteins were highly expressed in GTSs. Functional classification of these GSTs enriched proteins revealed that many of them participate in major plant metabolic processes such as electron transport, transcription and translation. Public Library of Science 2012-08-08 /pmc/articles/PMC3414485/ /pubmed/22905110 http://dx.doi.org/10.1371/journal.pone.0041822 Text en © 2012 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wu, Ting
Wang, Yejun
Guo, Dianjing
Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
title Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
title_full Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
title_fullStr Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
title_full_unstemmed Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
title_short Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
title_sort investigation of glandular trichome proteins in artemisia annua l. using comparative proteomics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414485/
https://www.ncbi.nlm.nih.gov/pubmed/22905110
http://dx.doi.org/10.1371/journal.pone.0041822
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