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Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics
Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414485/ https://www.ncbi.nlm.nih.gov/pubmed/22905110 http://dx.doi.org/10.1371/journal.pone.0041822 |
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author | Wu, Ting Wang, Yejun Guo, Dianjing |
author_facet | Wu, Ting Wang, Yejun Guo, Dianjing |
author_sort | Wu, Ting |
collection | PubMed |
description | Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%∼1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism. 2D-electrophoresis was employed to compare the protein profiles of GSTs and leaves. More than 700 spots were resolved for GSTs, of which ∼93 non-redundant proteins were confidently identified by searching NCBI and Artemisia EST databases. Over 70% of these proteins were highly expressed in GTSs. Functional classification of these GSTs enriched proteins revealed that many of them participate in major plant metabolic processes such as electron transport, transcription and translation. |
format | Online Article Text |
id | pubmed-3414485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34144852012-08-19 Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics Wu, Ting Wang, Yejun Guo, Dianjing PLoS One Research Article Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%∼1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism. 2D-electrophoresis was employed to compare the protein profiles of GSTs and leaves. More than 700 spots were resolved for GSTs, of which ∼93 non-redundant proteins were confidently identified by searching NCBI and Artemisia EST databases. Over 70% of these proteins were highly expressed in GTSs. Functional classification of these GSTs enriched proteins revealed that many of them participate in major plant metabolic processes such as electron transport, transcription and translation. Public Library of Science 2012-08-08 /pmc/articles/PMC3414485/ /pubmed/22905110 http://dx.doi.org/10.1371/journal.pone.0041822 Text en © 2012 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Ting Wang, Yejun Guo, Dianjing Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics |
title | Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics |
title_full | Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics |
title_fullStr | Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics |
title_full_unstemmed | Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics |
title_short | Investigation of Glandular Trichome Proteins in Artemisia annua L. Using Comparative Proteomics |
title_sort | investigation of glandular trichome proteins in artemisia annua l. using comparative proteomics |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414485/ https://www.ncbi.nlm.nih.gov/pubmed/22905110 http://dx.doi.org/10.1371/journal.pone.0041822 |
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