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The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing

The conserved Translin-TRAX complexes, also known as C3PO, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoribonuclease that promotes RNA interference in animal cells. Here we show that C3PO does not play a significant r...

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Detalles Bibliográficos
Autores principales: Li, Liande, Gu, Weifeng, Liang, Chunyang, Liu, Qinghua, Mello, Craig C., Liu, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414638/
https://www.ncbi.nlm.nih.gov/pubmed/22773104
http://dx.doi.org/10.1038/nsmb.2337
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author Li, Liande
Gu, Weifeng
Liang, Chunyang
Liu, Qinghua
Mello, Craig C.
Liu, Yi
author_facet Li, Liande
Gu, Weifeng
Liang, Chunyang
Liu, Qinghua
Mello, Craig C.
Liu, Yi
author_sort Li, Liande
collection PubMed
description The conserved Translin-TRAX complexes, also known as C3PO, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoribonuclease that promotes RNA interference in animal cells. Here we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as a ribonuclease that removes the 5′ pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, the translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell-death inducing agent. Finally, we showed that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. Together, this study identified the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in seemingly diverse biological processes.
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spelling pubmed-34146382013-02-01 The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing Li, Liande Gu, Weifeng Liang, Chunyang Liu, Qinghua Mello, Craig C. Liu, Yi Nat Struct Mol Biol Article The conserved Translin-TRAX complexes, also known as C3PO, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoribonuclease that promotes RNA interference in animal cells. Here we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as a ribonuclease that removes the 5′ pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, the translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell-death inducing agent. Finally, we showed that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. Together, this study identified the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in seemingly diverse biological processes. 2012-07-08 2012-08 /pmc/articles/PMC3414638/ /pubmed/22773104 http://dx.doi.org/10.1038/nsmb.2337 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Li, Liande
Gu, Weifeng
Liang, Chunyang
Liu, Qinghua
Mello, Craig C.
Liu, Yi
The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
title The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
title_full The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
title_fullStr The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
title_full_unstemmed The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
title_short The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
title_sort translin-trax complex/c3po is a ribonuclease in trna processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414638/
https://www.ncbi.nlm.nih.gov/pubmed/22773104
http://dx.doi.org/10.1038/nsmb.2337
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