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Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation
BACKGROUND: Cell migration plays an essential role in many biological processes, such as cancer metastasis, wound healing and immune response. Cell migration is mediated through protrusion and focal adhesion (FA) assembly, maturation and disassembly. Epidermal growth factor (EGF) is known to enhance...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414788/ https://www.ncbi.nlm.nih.gov/pubmed/22577847 http://dx.doi.org/10.1186/2046-1682-5-8 |
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author | Hou, Yue Hedberg, Sarah Schneider, Ian C |
author_facet | Hou, Yue Hedberg, Sarah Schneider, Ian C |
author_sort | Hou, Yue |
collection | PubMed |
description | BACKGROUND: Cell migration plays an essential role in many biological processes, such as cancer metastasis, wound healing and immune response. Cell migration is mediated through protrusion and focal adhesion (FA) assembly, maturation and disassembly. Epidermal growth factor (EGF) is known to enhance migration rate in many cell types; however it is not known how FA maturation, FA dynamics and protrusion dynamics are regulated during EGF-induced migration. Here we use total internal reflection fluorescence (TIRF) microscopy and image analysis to quantify FA properties and protrusion dynamics under different doses of EGF stimulation. RESULTS: EGF was found to broaden the distribution of cell migration rates, generating more fast and slow cells. Furthermore, groups based on EGF stimulation condition or cell migration speed were marked by characteristic signatures. When data was binned based on EGF stimulation conditions, FA intensity and FA number per cell showed the largest difference among stimulation groups. FA intensity decreased with increasing EGF concentration and FA number per cell was highest under intermediate stimulation conditions. No difference in protrusion behavior was observed. However, when data was binned based on cell migration speed, FA intensity and not FA number per cell showed the largest difference among groups. FA intensity was lower for fast migrating cells. Additionally, waves of protrusion tended to correlate with fast migrating cells. CONCLUSIONS: Only a portion of the FA properties and protrusion dynamics that correlate with migration speed, correlate with EGF stimulation condition. Those that do not correlate with EGF stimulation condition constitute the most sensitive output for identifying why cells respond differently to EGF. The idea that EGF can both increase and decrease the migration speed of individual cells in a population has particular relevance to cancer metastasis where the microenvironment can select subpopulations based on some adhesion and protrusion characteristics, leading to a more invasive phenotype as would be seen if all cells responded like an “average” cell. |
format | Online Article Text |
id | pubmed-3414788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34147882012-08-13 Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation Hou, Yue Hedberg, Sarah Schneider, Ian C BMC Biophys Research Article BACKGROUND: Cell migration plays an essential role in many biological processes, such as cancer metastasis, wound healing and immune response. Cell migration is mediated through protrusion and focal adhesion (FA) assembly, maturation and disassembly. Epidermal growth factor (EGF) is known to enhance migration rate in many cell types; however it is not known how FA maturation, FA dynamics and protrusion dynamics are regulated during EGF-induced migration. Here we use total internal reflection fluorescence (TIRF) microscopy and image analysis to quantify FA properties and protrusion dynamics under different doses of EGF stimulation. RESULTS: EGF was found to broaden the distribution of cell migration rates, generating more fast and slow cells. Furthermore, groups based on EGF stimulation condition or cell migration speed were marked by characteristic signatures. When data was binned based on EGF stimulation conditions, FA intensity and FA number per cell showed the largest difference among stimulation groups. FA intensity decreased with increasing EGF concentration and FA number per cell was highest under intermediate stimulation conditions. No difference in protrusion behavior was observed. However, when data was binned based on cell migration speed, FA intensity and not FA number per cell showed the largest difference among groups. FA intensity was lower for fast migrating cells. Additionally, waves of protrusion tended to correlate with fast migrating cells. CONCLUSIONS: Only a portion of the FA properties and protrusion dynamics that correlate with migration speed, correlate with EGF stimulation condition. Those that do not correlate with EGF stimulation condition constitute the most sensitive output for identifying why cells respond differently to EGF. The idea that EGF can both increase and decrease the migration speed of individual cells in a population has particular relevance to cancer metastasis where the microenvironment can select subpopulations based on some adhesion and protrusion characteristics, leading to a more invasive phenotype as would be seen if all cells responded like an “average” cell. BioMed Central 2012-05-11 /pmc/articles/PMC3414788/ /pubmed/22577847 http://dx.doi.org/10.1186/2046-1682-5-8 Text en Copyright ©2012 Hou et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hou, Yue Hedberg, Sarah Schneider, Ian C Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation |
title | Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation |
title_full | Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation |
title_fullStr | Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation |
title_full_unstemmed | Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation |
title_short | Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation |
title_sort | differences in adhesion and protrusion properties correlate with differences in migration speed under egf stimulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414788/ https://www.ncbi.nlm.nih.gov/pubmed/22577847 http://dx.doi.org/10.1186/2046-1682-5-8 |
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