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Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs in serum or plasma has been associated with many diseases includin...

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Detalles Bibliográficos
Autores principales: Kang, Kang, Peng, Xiao, Luo, Jun, Gou, Deming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415128/
https://www.ncbi.nlm.nih.gov/pubmed/22958414
http://dx.doi.org/10.1186/2049-1891-3-4
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author Kang, Kang
Peng, Xiao
Luo, Jun
Gou, Deming
author_facet Kang, Kang
Peng, Xiao
Luo, Jun
Gou, Deming
author_sort Kang, Kang
collection PubMed
description MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs in serum or plasma has been associated with many diseases including cancers and cardiovascular diseases, suggesting the possible use of miRNAs as diagnostic biomarkers. However, the detection of the small amount of miRNAs found in serum or plasma requires a method with high sensitivity and accuracy. Therefore, the current study describes polymerase chain reaction (PCR)-based methods for measuring circulating miRNAs. Briefly, the procedure involves four major steps: (1) sample collection and preparation; (2) global miRNAs profiling using quantitative real-time PCR (qRT-PCR); (3) data normalization and analysis; and (4) selection and validation of miRNA biomarkers. In conclusion, qRT-PCR is a promising method for profiling of circulating miRNAs as biomarkers.
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spelling pubmed-34151282012-08-13 Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling Kang, Kang Peng, Xiao Luo, Jun Gou, Deming J Anim Sci Biotechnol Review MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs in serum or plasma has been associated with many diseases including cancers and cardiovascular diseases, suggesting the possible use of miRNAs as diagnostic biomarkers. However, the detection of the small amount of miRNAs found in serum or plasma requires a method with high sensitivity and accuracy. Therefore, the current study describes polymerase chain reaction (PCR)-based methods for measuring circulating miRNAs. Briefly, the procedure involves four major steps: (1) sample collection and preparation; (2) global miRNAs profiling using quantitative real-time PCR (qRT-PCR); (3) data normalization and analysis; and (4) selection and validation of miRNA biomarkers. In conclusion, qRT-PCR is a promising method for profiling of circulating miRNAs as biomarkers. BioMed Central 2012-02-28 /pmc/articles/PMC3415128/ /pubmed/22958414 http://dx.doi.org/10.1186/2049-1891-3-4 Text en Copyright ©2012 Kang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Kang, Kang
Peng, Xiao
Luo, Jun
Gou, Deming
Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
title Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
title_full Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
title_fullStr Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
title_full_unstemmed Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
title_short Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
title_sort identification of circulating mirna biomarkers based on global quantitative real-time pcr profiling
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415128/
https://www.ncbi.nlm.nih.gov/pubmed/22958414
http://dx.doi.org/10.1186/2049-1891-3-4
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