FRAP Analysis: Accounting for Bleaching during Image Capture

The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleachin...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jun, Shekhar, Nandini, Lele, Pushkar P., Lele, Tanmay P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415426/
https://www.ncbi.nlm.nih.gov/pubmed/22912750
http://dx.doi.org/10.1371/journal.pone.0042854
_version_ 1782240359520141312
author Wu, Jun
Shekhar, Nandini
Lele, Pushkar P.
Lele, Tanmay P.
author_facet Wu, Jun
Shekhar, Nandini
Lele, Pushkar P.
Lele, Tanmay P.
author_sort Wu, Jun
collection PubMed
description The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.
format Online
Article
Text
id pubmed-3415426
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34154262012-08-21 FRAP Analysis: Accounting for Bleaching during Image Capture Wu, Jun Shekhar, Nandini Lele, Pushkar P. Lele, Tanmay P. PLoS One Research Article The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis. Public Library of Science 2012-08-09 /pmc/articles/PMC3415426/ /pubmed/22912750 http://dx.doi.org/10.1371/journal.pone.0042854 Text en © 2012 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wu, Jun
Shekhar, Nandini
Lele, Pushkar P.
Lele, Tanmay P.
FRAP Analysis: Accounting for Bleaching during Image Capture
title FRAP Analysis: Accounting for Bleaching during Image Capture
title_full FRAP Analysis: Accounting for Bleaching during Image Capture
title_fullStr FRAP Analysis: Accounting for Bleaching during Image Capture
title_full_unstemmed FRAP Analysis: Accounting for Bleaching during Image Capture
title_short FRAP Analysis: Accounting for Bleaching during Image Capture
title_sort frap analysis: accounting for bleaching during image capture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415426/
https://www.ncbi.nlm.nih.gov/pubmed/22912750
http://dx.doi.org/10.1371/journal.pone.0042854
work_keys_str_mv AT wujun frapanalysisaccountingforbleachingduringimagecapture
AT shekharnandini frapanalysisaccountingforbleachingduringimagecapture
AT lelepushkarp frapanalysisaccountingforbleachingduringimagecapture
AT leletanmayp frapanalysisaccountingforbleachingduringimagecapture

Ejemplares similares