Cargando…

FRAP Analysis: Accounting for Bleaching during Image Capture

The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleachin...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wu, Jun, Shekhar, Nandini, Lele, Pushkar P., Lele, Tanmay P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415426/ https://www.ncbi.nlm.nih.gov/pubmed/22912750 http://dx.doi.org/10.1371/journal.pone.0042854

Ejemplares similares

Universal Approach to FRAP Analysis of Arbitrary Bleaching Patterns
por: Blumenthal, Daniel, et al.
Publicado: (2015)

Evaluation of Pulsed-FRAP and Conventional-FRAP for Determination of Protein Mobility in Prokaryotic Cells
por: Mika, Jacek T., et al.
Publicado: (2011)

Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
por: Antani, Jyot D, et al.
Publicado: (2021)

Validation of Normalizations, Scaling, and Photofading Corrections for FRAP Data Analysis
por: Kang, Minchul, et al.
Publicado: (2015)

FRAP Analysis Reveals Stabilization of Adhesion Structures in the Epidermis Compared to Cultured Keratinocytes
por: Foote, Henry P., et al.
Publicado: (2013)

Mobility of kinetochore proteins measured by FRAP analysis in living cells
por: Watanabe, Reito, et al.
Publicado: (2022)

In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP
por: Gaume, Xavier, et al.
Publicado: (2011)

Photoswitching-Free FRAP Analysis with a Genetically Encoded Fluorescent Tag
por: Morisaki, Tatsuya, et al.
Publicado: (2014)

Confocal Fluorescence Anisotropy and FRAP Imaging of α-Synuclein Amyloid Aggregates in Living Cells
por: Roberti, M. Julia, et al.
Publicado: (2011)

FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments
por: Pincet, Frédéric, et al.
Publicado: (2016)

FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development
por: Ooga, Masatoshi, et al.
Publicado: (2017)

Protein expression-independent response of intensity-based pH-sensitive fluorophores in Escherichia coli
por: Rhee, Kathy Y., et al.
Publicado: (2020)

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
por: Ishikawa-Ankerhold, Hellen C., et al.
Publicado: (2012)

Quantitation of Glucocorticoid Receptor DNA-Binding Dynamics by Single-Molecule Microscopy and FRAP
por: Groeneweg, Femke L., et al.
Publicado: (2014)

Approach to Optimization of FRAP Methodology for Studies Based on Selected Monoterpenes
por: Wojtunik-Kulesza, Karolina A.
Publicado: (2020)

In vivo dynamics of skeletal muscle Dystrophin in zebrafish embryos revealed by improved FRAP analysis
por: Bajanca, Fernanda, et al.
Publicado: (2015)

Oxidative stress and biomarker of TNF-α, MDA and FRAP in hypertension
por: Verma, Manish Kumar, et al.
Publicado: (2019)

Bacterial Proprioception: Can a Bacterium Sense Its Movement?
por: Gupta, Rachit, et al.
Publicado: (2022)

Theoretical model of mitotic spindle microtubule growth for FRAP curve interpretation
por: Omelyanchuk, Leonid V., et al.
Publicado: (2017)

Concentration Sensing by the Moving Nucleus in Cell Fate Determination: A Computational Analysis
por: Aggarwal, Varun, et al.
Publicado: (2016)

Protocol for detecting chromatin dynamics and screening chromatin relaxer by FRAP assay
por: Long, Qi, et al.
Publicado: (2021)

Torque, but not FliL, regulates mechanosensitive flagellar motor-function
por: Chawla, Ravi, et al.
Publicado: (2017)

Geometry of the nuclear envelope determines its flexural stiffness
por: Agrawal, Ashutosh, et al.
Publicado: (2020)

Distinct modes of perimembrane TRP channel turnover revealed by TIR-FRAP
por: Ghosh, Debapriya, et al.
Publicado: (2014)

Quantitative diffusion measurements using the open-source software PyFRAP
por: Bläßle, Alexander, et al.
Publicado: (2018)

Salivary FRAP as A Marker of Chronic Kidney Disease Progression in Children
por: Maciejczyk, Mateusz, et al.
Publicado: (2019)

Response thresholds in bacterial chemotaxis
por: Lele, Pushkar P., et al.
Publicado: (2015)

Direct Measurement of Diffusion in Olfactory Cilia Using a Modified FRAP Approach
por: Alevra, Mihai, et al.
Publicado: (2012)

Periodontal cell mechanotransduction
por: Chukkapalli, Sasanka S., et al.
Publicado: (2018)

The Nucleus Bypasses Obstacles by Deforming Like a Drop with Surface Tension Mediated by Lamin A/C
por: Katiyar, Aditya, et al.
Publicado: (2022)

The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane
por: Day, Charles A., et al.
Publicado: (2023)

Feasibility Analysis of Polyurethane-Prepolymer-Modified Bitumen Used for Fully Reclaimed Asphalt Pavement (FRAP)
por: Sun, Minggang, et al.
Publicado: (2023)

Analyses of In Vivo Interaction and Mobility of Two Spliceosomal Proteins Using FRAP and BiFC
por: Ali, Gul Shad, et al.
Publicado: (2008)

Nuclear shapes are geometrically determined by the excess surface area of the nuclear lamina
por: Dickinson, Richard B., et al.
Publicado: (2023)

Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules
por: Rayan, Gamal, et al.
Publicado: (2010)

Boron Neutron Capture Therapy: Current Status and Challenges
por: Wang, Song, et al.
Publicado: (2022)

DeepFRAP: Fast fluorescence recovery after photobleaching data analysis using deep neural networks
por: Wåhlstrand Skärström, Victor, et al.
Publicado: (2021)

Editorial: Biological rotary nanomotors
por: Manson, Michael D., et al.
Publicado: (2022)

The role of glycans in the mechanobiology of cancer
por: Purushothaman, Anurag, et al.
Publicado: (2023)

EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data
por: Koulouras, Grigorios, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_ke9f1uihhmuci6j7vf1f67g23c