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Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA

Cohesin is a protein complex that forms a ring around sister chromatids thus holding them together. The ring is composed of three proteins: Smc1, Smc3 and Scc1. The roles of three additional proteins that associate with the ring, Scc3, Pds5 and Wpl1, are not well understood. It has been proposed tha...

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Autores principales: Kulemzina, Irina, Schumacher, Martin R., Verma, Vikash, Reiter, Jochen, Metzler, Janina, Failla, Antonio Virgilio, Lanz, Christa, Sreedharan, Vipin T., Rätsch, Gunnar, Ivanov, Dmitri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415457/
https://www.ncbi.nlm.nih.gov/pubmed/22912589
http://dx.doi.org/10.1371/journal.pgen.1002856
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author Kulemzina, Irina
Schumacher, Martin R.
Verma, Vikash
Reiter, Jochen
Metzler, Janina
Failla, Antonio Virgilio
Lanz, Christa
Sreedharan, Vipin T.
Rätsch, Gunnar
Ivanov, Dmitri
author_facet Kulemzina, Irina
Schumacher, Martin R.
Verma, Vikash
Reiter, Jochen
Metzler, Janina
Failla, Antonio Virgilio
Lanz, Christa
Sreedharan, Vipin T.
Rätsch, Gunnar
Ivanov, Dmitri
author_sort Kulemzina, Irina
collection PubMed
description Cohesin is a protein complex that forms a ring around sister chromatids thus holding them together. The ring is composed of three proteins: Smc1, Smc3 and Scc1. The roles of three additional proteins that associate with the ring, Scc3, Pds5 and Wpl1, are not well understood. It has been proposed that these three factors form a complex that stabilizes the ring and prevents it from opening. This activity promotes sister chromatid cohesion but at the same time poses an obstacle for the initial entrapment of sister DNAs. This hindrance to cohesion establishment is overcome during DNA replication via acetylation of the Smc3 subunit by the Eco1 acetyltransferase. However, the full mechanistic consequences of Smc3 acetylation remain unknown. In the current work, we test the requirement of Scc3 and Pds5 for the stable association of cohesin with DNA. We investigated the consequences of Scc3 and Pds5 depletion in vivo using degron tagging in budding yeast. The previously described DHFR–based N-terminal degron as well as a novel Eco1-derived C-terminal degron were employed in our study. Scc3 and Pds5 associate with cohesin complexes independently of each other and require the Scc1 “core” subunit for their association with chromosomes. Contrary to previous data for Scc1 downregulation, depletion of either Scc3 or Pds5 had a strong effect on sister chromatid cohesion but not on cohesin binding to DNA. Quantity, stability and genome-wide distribution of cohesin complexes remained mostly unchanged after the depletion of Scc3 and Pds5. Our findings are inconsistent with a previously proposed model that Scc3 and Pds5 are cohesin maintenance factors required for cohesin ring stability or for maintaining its association with DNA. We propose that Scc3 and Pds5 specifically function during cohesion establishment in S phase.
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spelling pubmed-34154572012-08-21 Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA Kulemzina, Irina Schumacher, Martin R. Verma, Vikash Reiter, Jochen Metzler, Janina Failla, Antonio Virgilio Lanz, Christa Sreedharan, Vipin T. Rätsch, Gunnar Ivanov, Dmitri PLoS Genet Research Article Cohesin is a protein complex that forms a ring around sister chromatids thus holding them together. The ring is composed of three proteins: Smc1, Smc3 and Scc1. The roles of three additional proteins that associate with the ring, Scc3, Pds5 and Wpl1, are not well understood. It has been proposed that these three factors form a complex that stabilizes the ring and prevents it from opening. This activity promotes sister chromatid cohesion but at the same time poses an obstacle for the initial entrapment of sister DNAs. This hindrance to cohesion establishment is overcome during DNA replication via acetylation of the Smc3 subunit by the Eco1 acetyltransferase. However, the full mechanistic consequences of Smc3 acetylation remain unknown. In the current work, we test the requirement of Scc3 and Pds5 for the stable association of cohesin with DNA. We investigated the consequences of Scc3 and Pds5 depletion in vivo using degron tagging in budding yeast. The previously described DHFR–based N-terminal degron as well as a novel Eco1-derived C-terminal degron were employed in our study. Scc3 and Pds5 associate with cohesin complexes independently of each other and require the Scc1 “core” subunit for their association with chromosomes. Contrary to previous data for Scc1 downregulation, depletion of either Scc3 or Pds5 had a strong effect on sister chromatid cohesion but not on cohesin binding to DNA. Quantity, stability and genome-wide distribution of cohesin complexes remained mostly unchanged after the depletion of Scc3 and Pds5. Our findings are inconsistent with a previously proposed model that Scc3 and Pds5 are cohesin maintenance factors required for cohesin ring stability or for maintaining its association with DNA. We propose that Scc3 and Pds5 specifically function during cohesion establishment in S phase. Public Library of Science 2012-08-09 /pmc/articles/PMC3415457/ /pubmed/22912589 http://dx.doi.org/10.1371/journal.pgen.1002856 Text en © 2012 Kulemzina et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kulemzina, Irina
Schumacher, Martin R.
Verma, Vikash
Reiter, Jochen
Metzler, Janina
Failla, Antonio Virgilio
Lanz, Christa
Sreedharan, Vipin T.
Rätsch, Gunnar
Ivanov, Dmitri
Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA
title Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA
title_full Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA
title_fullStr Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA
title_full_unstemmed Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA
title_short Cohesin Rings Devoid of Scc3 and Pds5 Maintain Their Stable Association with the DNA
title_sort cohesin rings devoid of scc3 and pds5 maintain their stable association with the dna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415457/
https://www.ncbi.nlm.nih.gov/pubmed/22912589
http://dx.doi.org/10.1371/journal.pgen.1002856
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