Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDR...

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Detalles Bibliográficos
Autores principales: Morlan, John D., Qu, Kunbin, Sinicropi, Dominick V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416766/
https://www.ncbi.nlm.nih.gov/pubmed/22900061
http://dx.doi.org/10.1371/journal.pone.0042882
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author Morlan, John D.
Qu, Kunbin
Sinicropi, Dominick V.
author_facet Morlan, John D.
Qu, Kunbin
Sinicropi, Dominick V.
author_sort Morlan, John D.
collection PubMed
description We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.
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spelling pubmed-34167662012-08-16 Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue Morlan, John D. Qu, Kunbin Sinicropi, Dominick V. PLoS One Research Article We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing. Public Library of Science 2012-08-10 /pmc/articles/PMC3416766/ /pubmed/22900061 http://dx.doi.org/10.1371/journal.pone.0042882 Text en © 2012 Morlan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Morlan, John D.
Qu, Kunbin
Sinicropi, Dominick V.
Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue
title Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue
title_full Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue
title_fullStr Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue
title_full_unstemmed Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue
title_short Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue
title_sort selective depletion of rrna enables whole transcriptome profiling of archival fixed tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416766/
https://www.ncbi.nlm.nih.gov/pubmed/22900061
http://dx.doi.org/10.1371/journal.pone.0042882
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