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CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients

BACKGROUND: Neuroblastoma is thought to originate from neural crest-derived cells. CD57 defines migratory neural crest cells in normal development and is expressed in neuroblastoma. METHODOLOGY AND PRINCIPAL FINDINGS: We investigated the role of CD57 expression in neuroblastoma cells ex situ and in...

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Autores principales: Schlitter, Anne-Marie, Dorneburg, Carmen, Barth, Thomas F. E., Wahl, Joachim, Schulte, Johannes H., Brüderlein, Silke, Debatin, Klaus-Michael, Beltinger, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416815/
https://www.ncbi.nlm.nih.gov/pubmed/22900004
http://dx.doi.org/10.1371/journal.pone.0042025
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author Schlitter, Anne-Marie
Dorneburg, Carmen
Barth, Thomas F. E.
Wahl, Joachim
Schulte, Johannes H.
Brüderlein, Silke
Debatin, Klaus-Michael
Beltinger, Christian
author_facet Schlitter, Anne-Marie
Dorneburg, Carmen
Barth, Thomas F. E.
Wahl, Joachim
Schulte, Johannes H.
Brüderlein, Silke
Debatin, Klaus-Michael
Beltinger, Christian
author_sort Schlitter, Anne-Marie
collection PubMed
description BACKGROUND: Neuroblastoma is thought to originate from neural crest-derived cells. CD57 defines migratory neural crest cells in normal development and is expressed in neuroblastoma. METHODOLOGY AND PRINCIPAL FINDINGS: We investigated the role of CD57 expression in neuroblastoma cells ex situ and in situ. Compared to CD57(low) U-NB1 neuroblastoma cells, CD57(high) cells developed tumors with decreased latency after orthotopic transplantation into adrenal glands of mice. In addition, CD57(high) U-NB1 and SK-N-BE(2)-C neuroblastoma cells were also more clonogenic, induced more spheres and were less lineage-restricted. CD57(high) cells attached better to endothelial cells and showed enhanced invasiveness. While invasion of U-NB1 cells was inhibited by blocking antibodies against CD57, neither invasion of SK-N-BE(2)-C cells nor adhesion of U-NB1 and SK-N-BE(2)-C cells was attenuated. After tail vein injection only CD57(high) cells generated liver metastases, while overall metastatic rate was not increased as compared to CD57(low) cells. In stroma-poor neuroblastoma of patients CD57(high) cells were associated with undifferentiated tumor cells across all stages and tended to be more frequent after chemotherapy. CONCLUSION: Strong expression of CD57 correlates with aggressive attributes of U-NB1 and SK-N-BE(2)-C neuroblastoma cells and is linked with undifferentiated neuroblastoma cells in patients.
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spelling pubmed-34168152012-08-16 CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients Schlitter, Anne-Marie Dorneburg, Carmen Barth, Thomas F. E. Wahl, Joachim Schulte, Johannes H. Brüderlein, Silke Debatin, Klaus-Michael Beltinger, Christian PLoS One Research Article BACKGROUND: Neuroblastoma is thought to originate from neural crest-derived cells. CD57 defines migratory neural crest cells in normal development and is expressed in neuroblastoma. METHODOLOGY AND PRINCIPAL FINDINGS: We investigated the role of CD57 expression in neuroblastoma cells ex situ and in situ. Compared to CD57(low) U-NB1 neuroblastoma cells, CD57(high) cells developed tumors with decreased latency after orthotopic transplantation into adrenal glands of mice. In addition, CD57(high) U-NB1 and SK-N-BE(2)-C neuroblastoma cells were also more clonogenic, induced more spheres and were less lineage-restricted. CD57(high) cells attached better to endothelial cells and showed enhanced invasiveness. While invasion of U-NB1 cells was inhibited by blocking antibodies against CD57, neither invasion of SK-N-BE(2)-C cells nor adhesion of U-NB1 and SK-N-BE(2)-C cells was attenuated. After tail vein injection only CD57(high) cells generated liver metastases, while overall metastatic rate was not increased as compared to CD57(low) cells. In stroma-poor neuroblastoma of patients CD57(high) cells were associated with undifferentiated tumor cells across all stages and tended to be more frequent after chemotherapy. CONCLUSION: Strong expression of CD57 correlates with aggressive attributes of U-NB1 and SK-N-BE(2)-C neuroblastoma cells and is linked with undifferentiated neuroblastoma cells in patients. Public Library of Science 2012-08-10 /pmc/articles/PMC3416815/ /pubmed/22900004 http://dx.doi.org/10.1371/journal.pone.0042025 Text en © 2012 Schlitter et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schlitter, Anne-Marie
Dorneburg, Carmen
Barth, Thomas F. E.
Wahl, Joachim
Schulte, Johannes H.
Brüderlein, Silke
Debatin, Klaus-Michael
Beltinger, Christian
CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients
title CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients
title_full CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients
title_fullStr CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients
title_full_unstemmed CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients
title_short CD57(high) Neuroblastoma Cells Have Aggressive Attributes Ex Situ and an Undifferentiated Phenotype in Patients
title_sort cd57(high) neuroblastoma cells have aggressive attributes ex situ and an undifferentiated phenotype in patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416815/
https://www.ncbi.nlm.nih.gov/pubmed/22900004
http://dx.doi.org/10.1371/journal.pone.0042025
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