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The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain

In Australia the PVL - positive ST93-IV [2B], colloquially known as “Queensland CA-MRSA” has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence...

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Autores principales: Coombs, Geoffrey W., Goering, Richard V., Chua, Kyra Y. L., Monecke, Stefan, Howden, Benjamin P., Stinear, Timothy P., Ehricht, Ralf, O’Brien, Frances G., Christiansen, Keryn J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416834/
https://www.ncbi.nlm.nih.gov/pubmed/22900085
http://dx.doi.org/10.1371/journal.pone.0043037
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author Coombs, Geoffrey W.
Goering, Richard V.
Chua, Kyra Y. L.
Monecke, Stefan
Howden, Benjamin P.
Stinear, Timothy P.
Ehricht, Ralf
O’Brien, Frances G.
Christiansen, Keryn J.
author_facet Coombs, Geoffrey W.
Goering, Richard V.
Chua, Kyra Y. L.
Monecke, Stefan
Howden, Benjamin P.
Stinear, Timothy P.
Ehricht, Ralf
O’Brien, Frances G.
Christiansen, Keryn J.
author_sort Coombs, Geoffrey W.
collection PubMed
description In Australia the PVL - positive ST93-IV [2B], colloquially known as “Queensland CA-MRSA” has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence typing (MLST) eBURST analysis ST93 is distinct from other S aureus clones. To determine if the increased prevalence of ST93-IV [2B] is due to the widespread transmission of a single strain of ST93-IV [2B] the genetic relatedness of 58 S. aureus ST93 isolated throughout Australia over an extended period were studied in detail using a variety of molecular methods including pulsed-field gel electrophoresis, spa typing, MLST, microarray DNA, SCCmec typing and dru typing. Identification of the phage harbouring the lukS-PV/lukF-PV Panton Valentine leucocidin genes, detection of allelic variations in lukS-PV/lukF-PV, and quantification of LukF-PV expression was also performed. Although ST93-IV [2B] is known to have an apparent enhanced clinical virulence, the isolates harboured few known virulence determinants. All PVL-positive isolates carried the PVL-encoding phage ΦSa2USA and the lukS-PV/lukF-PV genes had the same R variant SNP profile. The isolates produced similar expression levels of LukF-PV. Although multiple rearrangements of the spa sequence have occurred, the core genome in ST93 is very stable. The emergence of ST93-MRSA is due to independent acquisitions of different dru-defined type IV and type V SCCmec elements in several spa-defined ST93-MSSA backgrounds. Rearrangement of the spa sequence in ST93-MRSA has subsequently occurred in some of these strains. Although multiple ST93-MRSA strains were characterised, little genetic diversity was identified for most isolates, with PVL-positive ST93-IVa [2B]-t202-dt10 predominant across Australia. Whether ST93-IVa [2B] t202-dt10 arose from one PVL-positive ST93-MSSA-t202, or by independent acquisitions of SCCmec-IVa [2B]-dt10 into multiple PVL-positive ST93-MSSA-t202 strains is not known.
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spelling pubmed-34168342012-08-16 The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain Coombs, Geoffrey W. Goering, Richard V. Chua, Kyra Y. L. Monecke, Stefan Howden, Benjamin P. Stinear, Timothy P. Ehricht, Ralf O’Brien, Frances G. Christiansen, Keryn J. PLoS One Research Article In Australia the PVL - positive ST93-IV [2B], colloquially known as “Queensland CA-MRSA” has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence typing (MLST) eBURST analysis ST93 is distinct from other S aureus clones. To determine if the increased prevalence of ST93-IV [2B] is due to the widespread transmission of a single strain of ST93-IV [2B] the genetic relatedness of 58 S. aureus ST93 isolated throughout Australia over an extended period were studied in detail using a variety of molecular methods including pulsed-field gel electrophoresis, spa typing, MLST, microarray DNA, SCCmec typing and dru typing. Identification of the phage harbouring the lukS-PV/lukF-PV Panton Valentine leucocidin genes, detection of allelic variations in lukS-PV/lukF-PV, and quantification of LukF-PV expression was also performed. Although ST93-IV [2B] is known to have an apparent enhanced clinical virulence, the isolates harboured few known virulence determinants. All PVL-positive isolates carried the PVL-encoding phage ΦSa2USA and the lukS-PV/lukF-PV genes had the same R variant SNP profile. The isolates produced similar expression levels of LukF-PV. Although multiple rearrangements of the spa sequence have occurred, the core genome in ST93 is very stable. The emergence of ST93-MRSA is due to independent acquisitions of different dru-defined type IV and type V SCCmec elements in several spa-defined ST93-MSSA backgrounds. Rearrangement of the spa sequence in ST93-MRSA has subsequently occurred in some of these strains. Although multiple ST93-MRSA strains were characterised, little genetic diversity was identified for most isolates, with PVL-positive ST93-IVa [2B]-t202-dt10 predominant across Australia. Whether ST93-IVa [2B] t202-dt10 arose from one PVL-positive ST93-MSSA-t202, or by independent acquisitions of SCCmec-IVa [2B]-dt10 into multiple PVL-positive ST93-MSSA-t202 strains is not known. Public Library of Science 2012-08-10 /pmc/articles/PMC3416834/ /pubmed/22900085 http://dx.doi.org/10.1371/journal.pone.0043037 Text en © 2012 Coombs et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Coombs, Geoffrey W.
Goering, Richard V.
Chua, Kyra Y. L.
Monecke, Stefan
Howden, Benjamin P.
Stinear, Timothy P.
Ehricht, Ralf
O’Brien, Frances G.
Christiansen, Keryn J.
The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
title The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
title_full The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
title_fullStr The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
title_full_unstemmed The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
title_short The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
title_sort molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416834/
https://www.ncbi.nlm.nih.gov/pubmed/22900085
http://dx.doi.org/10.1371/journal.pone.0043037
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