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Thermal Stabilization of an Endoglucanase by Cyclization

An intein-driven protein splicing approach allowed for the covalent linkage between the N- and C-termini of a polypeptide chain to create circular variants of the endo-β-1,3-1,4-glucanase, LicA, from Bacillus licheniformis. Two circular variants, LicA-C1 and LicA-C2, which have connecting loops of 2...

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Detalles Bibliográficos
Autores principales:	van Lieshout, Johan F. T., Gutiérrez, Odette N. Pérez, Vroom, Wietse, Planas, Antoni, de Vos, Willem M., van der Oost, John, Koutsopoulos, Sotirios
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer-Verlag 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416981/ https://www.ncbi.nlm.nih.gov/pubmed/22653681 http://dx.doi.org/10.1007/s12010-012-9674-z

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