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Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement

Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movem...

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Autores principales: Ellias, Mohd Faiz, Zainal Ariffin, Shahrul Hisham, Karsani, Saiful Anuar, Abdul Rahman, Mariati, Senafi, Shahidan, Megat Abdul Wahab, Rohaya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417200/
https://www.ncbi.nlm.nih.gov/pubmed/22919344
http://dx.doi.org/10.1100/2012/647240
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author Ellias, Mohd Faiz
Zainal Ariffin, Shahrul Hisham
Karsani, Saiful Anuar
Abdul Rahman, Mariati
Senafi, Shahidan
Megat Abdul Wahab, Rohaya
author_facet Ellias, Mohd Faiz
Zainal Ariffin, Shahrul Hisham
Karsani, Saiful Anuar
Abdul Rahman, Mariati
Senafi, Shahidan
Megat Abdul Wahab, Rohaya
author_sort Ellias, Mohd Faiz
collection PubMed
description Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movement. These differentially expressed proteins can potentially serve as protein biomarkers for the monitoring of orthodontic treatment and tooth movement. Whole saliva from three healthy female subjects were collected before force application using fixed appliance and at 14 days after 0.014′′ Niti wire was applied. Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3–10, and the resulting proteome profiles were compared. Differentially expressed protein spots were then identified by MALDI-TOF/TOF tandem mass spectrometry. Nine proteins were found to be differentially expressed; however, only eight were identified by MALDI-TOF/TOF. Four of these proteins—Protein S100-A9, immunoglobulin J chain, Ig alpha-1 chain C region, and CRISP-3—have known roles in inflammation and bone resorption.
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spelling pubmed-34172002012-08-23 Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement Ellias, Mohd Faiz Zainal Ariffin, Shahrul Hisham Karsani, Saiful Anuar Abdul Rahman, Mariati Senafi, Shahidan Megat Abdul Wahab, Rohaya ScientificWorldJournal Research Article Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movement. These differentially expressed proteins can potentially serve as protein biomarkers for the monitoring of orthodontic treatment and tooth movement. Whole saliva from three healthy female subjects were collected before force application using fixed appliance and at 14 days after 0.014′′ Niti wire was applied. Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3–10, and the resulting proteome profiles were compared. Differentially expressed protein spots were then identified by MALDI-TOF/TOF tandem mass spectrometry. Nine proteins were found to be differentially expressed; however, only eight were identified by MALDI-TOF/TOF. Four of these proteins—Protein S100-A9, immunoglobulin J chain, Ig alpha-1 chain C region, and CRISP-3—have known roles in inflammation and bone resorption. The Scientific World Journal 2012-07-31 /pmc/articles/PMC3417200/ /pubmed/22919344 http://dx.doi.org/10.1100/2012/647240 Text en Copyright © 2012 Mohd Faiz Ellias et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ellias, Mohd Faiz
Zainal Ariffin, Shahrul Hisham
Karsani, Saiful Anuar
Abdul Rahman, Mariati
Senafi, Shahidan
Megat Abdul Wahab, Rohaya
Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
title Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
title_full Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
title_fullStr Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
title_full_unstemmed Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
title_short Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
title_sort proteomic analysis of saliva identifies potential biomarkers for orthodontic tooth movement
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417200/
https://www.ncbi.nlm.nih.gov/pubmed/22919344
http://dx.doi.org/10.1100/2012/647240
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