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Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movem...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific World Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417200/ https://www.ncbi.nlm.nih.gov/pubmed/22919344 http://dx.doi.org/10.1100/2012/647240 |
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author | Ellias, Mohd Faiz Zainal Ariffin, Shahrul Hisham Karsani, Saiful Anuar Abdul Rahman, Mariati Senafi, Shahidan Megat Abdul Wahab, Rohaya |
author_facet | Ellias, Mohd Faiz Zainal Ariffin, Shahrul Hisham Karsani, Saiful Anuar Abdul Rahman, Mariati Senafi, Shahidan Megat Abdul Wahab, Rohaya |
author_sort | Ellias, Mohd Faiz |
collection | PubMed |
description | Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movement. These differentially expressed proteins can potentially serve as protein biomarkers for the monitoring of orthodontic treatment and tooth movement. Whole saliva from three healthy female subjects were collected before force application using fixed appliance and at 14 days after 0.014′′ Niti wire was applied. Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3–10, and the resulting proteome profiles were compared. Differentially expressed protein spots were then identified by MALDI-TOF/TOF tandem mass spectrometry. Nine proteins were found to be differentially expressed; however, only eight were identified by MALDI-TOF/TOF. Four of these proteins—Protein S100-A9, immunoglobulin J chain, Ig alpha-1 chain C region, and CRISP-3—have known roles in inflammation and bone resorption. |
format | Online Article Text |
id | pubmed-3417200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Scientific World Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-34172002012-08-23 Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement Ellias, Mohd Faiz Zainal Ariffin, Shahrul Hisham Karsani, Saiful Anuar Abdul Rahman, Mariati Senafi, Shahidan Megat Abdul Wahab, Rohaya ScientificWorldJournal Research Article Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movement. These differentially expressed proteins can potentially serve as protein biomarkers for the monitoring of orthodontic treatment and tooth movement. Whole saliva from three healthy female subjects were collected before force application using fixed appliance and at 14 days after 0.014′′ Niti wire was applied. Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3–10, and the resulting proteome profiles were compared. Differentially expressed protein spots were then identified by MALDI-TOF/TOF tandem mass spectrometry. Nine proteins were found to be differentially expressed; however, only eight were identified by MALDI-TOF/TOF. Four of these proteins—Protein S100-A9, immunoglobulin J chain, Ig alpha-1 chain C region, and CRISP-3—have known roles in inflammation and bone resorption. The Scientific World Journal 2012-07-31 /pmc/articles/PMC3417200/ /pubmed/22919344 http://dx.doi.org/10.1100/2012/647240 Text en Copyright © 2012 Mohd Faiz Ellias et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ellias, Mohd Faiz Zainal Ariffin, Shahrul Hisham Karsani, Saiful Anuar Abdul Rahman, Mariati Senafi, Shahidan Megat Abdul Wahab, Rohaya Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
title | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
title_full | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
title_fullStr | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
title_full_unstemmed | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
title_short | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
title_sort | proteomic analysis of saliva identifies potential biomarkers for orthodontic tooth movement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417200/ https://www.ncbi.nlm.nih.gov/pubmed/22919344 http://dx.doi.org/10.1100/2012/647240 |
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