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Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens

The Staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including S. intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. S. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infectio...

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Autores principales: Ben Zakour, Nouri L., Beatson, Scott A., van den Broek, Adri H. M., Thoday, Keith L., Fitzgerald, J. Ross
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417630/
https://www.ncbi.nlm.nih.gov/pubmed/22919635
http://dx.doi.org/10.3389/fcimb.2012.00044
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author Ben Zakour, Nouri L.
Beatson, Scott A.
van den Broek, Adri H. M.
Thoday, Keith L.
Fitzgerald, J. Ross
author_facet Ben Zakour, Nouri L.
Beatson, Scott A.
van den Broek, Adri H. M.
Thoday, Keith L.
Fitzgerald, J. Ross
author_sort Ben Zakour, Nouri L.
collection PubMed
description The Staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including S. intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. S. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. S. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas S. intermedius has been isolated only from pigeons to date. Here we provide a detailed analysis of the S. pseudintermedius whole genome sequence in comparison to high quality draft S. intermedius and S. delphini genomes, and to other sequenced staphylococcal species. The core genome of the SIG was highly conserved with average nucleotide identity (ANI) between the three species of 93.61%, which is very close to the threshold of species delineation (95% ANI), highlighting the close-relatedness of the SIG species. However, considerable variation was identified in the content of mobile genetic elements, cell wall-associated proteins, and iron and sugar transporters, reflecting the distinct ecological niches inhabited. Of note, S. pseudintermedius ED99 contained a clustered regularly interspaced short palindromic repeat locus of the Nmeni subtype and S. intermedius contained both Nmeni and Mtube subtypes. In contrast to S. intermedius and S. delphini and most other staphylococci examined to date, S. pseudintermedius contained at least nine predicted reverse transcriptase Group II introns. Furthermore, S. pseudintermedius ED99 encoded several transposons which were largely responsible for its multi-resistant phenotype. Overall, the study highlights extensive differences in accessory genome content between closely related staphylococcal species inhabiting distinct host niches, providing new avenues for research into pathogenesis and bacterial host-adaptation.
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spelling pubmed-34176302012-08-23 Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens Ben Zakour, Nouri L. Beatson, Scott A. van den Broek, Adri H. M. Thoday, Keith L. Fitzgerald, J. Ross Front Cell Infect Microbiol Microbiology The Staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including S. intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. S. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. S. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas S. intermedius has been isolated only from pigeons to date. Here we provide a detailed analysis of the S. pseudintermedius whole genome sequence in comparison to high quality draft S. intermedius and S. delphini genomes, and to other sequenced staphylococcal species. The core genome of the SIG was highly conserved with average nucleotide identity (ANI) between the three species of 93.61%, which is very close to the threshold of species delineation (95% ANI), highlighting the close-relatedness of the SIG species. However, considerable variation was identified in the content of mobile genetic elements, cell wall-associated proteins, and iron and sugar transporters, reflecting the distinct ecological niches inhabited. Of note, S. pseudintermedius ED99 contained a clustered regularly interspaced short palindromic repeat locus of the Nmeni subtype and S. intermedius contained both Nmeni and Mtube subtypes. In contrast to S. intermedius and S. delphini and most other staphylococci examined to date, S. pseudintermedius contained at least nine predicted reverse transcriptase Group II introns. Furthermore, S. pseudintermedius ED99 encoded several transposons which were largely responsible for its multi-resistant phenotype. Overall, the study highlights extensive differences in accessory genome content between closely related staphylococcal species inhabiting distinct host niches, providing new avenues for research into pathogenesis and bacterial host-adaptation. Frontiers Research Foundation 2012-04-18 /pmc/articles/PMC3417630/ /pubmed/22919635 http://dx.doi.org/10.3389/fcimb.2012.00044 Text en Copyright © 2012 Ben Zakour, Beatson, van den Broek, Thoday and Fitzgerald. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
spellingShingle Microbiology
Ben Zakour, Nouri L.
Beatson, Scott A.
van den Broek, Adri H. M.
Thoday, Keith L.
Fitzgerald, J. Ross
Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens
title Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens
title_full Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens
title_fullStr Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens
title_full_unstemmed Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens
title_short Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens
title_sort comparative genomics of the staphylococcus intermedius group of animal pathogens
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417630/
https://www.ncbi.nlm.nih.gov/pubmed/22919635
http://dx.doi.org/10.3389/fcimb.2012.00044
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