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Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418172/ https://www.ncbi.nlm.nih.gov/pubmed/22915841 http://dx.doi.org/10.2147/IJN.S32681 |
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author | Tiryaki, Volkan Mujdat Ayres, Virginia M Khan, Adeel A Ahmed, Ijaz Shreiber, David I Meiners, Sally |
author_facet | Tiryaki, Volkan Mujdat Ayres, Virginia M Khan, Adeel A Ahmed, Ijaz Shreiber, David I Meiners, Sally |
author_sort | Tiryaki, Volkan Mujdat |
collection | PubMed |
description | Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical properties of the cell culture environments were evaluated using contact angle and surface roughness measurements and compared. Astrocyte morphological responses, including filopodia, lamellipodia, and stress fiber formation, and stellation were imaged using atomic force microscopy and phalloidin staining for F-actin. Activation of the corresponding Rho GTPase regulators was investigated using immunolabeling with Cdc42, Rac1, and RhoA. Astrocytes cultured on the nanofibrillar scaffolds showed a unique response that included stellation, cell–cell interactions by stellate processes, and evidence of depression of RhoA. The results support the hypothesis that the extracellular environment can trigger preferential activation of members of the Rho GTPase family, with demonstrable morphological consequences for cerebral cortical astrocytes. |
format | Online Article Text |
id | pubmed-3418172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34181722012-08-22 Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes Tiryaki, Volkan Mujdat Ayres, Virginia M Khan, Adeel A Ahmed, Ijaz Shreiber, David I Meiners, Sally Int J Nanomedicine Original Research Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical properties of the cell culture environments were evaluated using contact angle and surface roughness measurements and compared. Astrocyte morphological responses, including filopodia, lamellipodia, and stress fiber formation, and stellation were imaged using atomic force microscopy and phalloidin staining for F-actin. Activation of the corresponding Rho GTPase regulators was investigated using immunolabeling with Cdc42, Rac1, and RhoA. Astrocytes cultured on the nanofibrillar scaffolds showed a unique response that included stellation, cell–cell interactions by stellate processes, and evidence of depression of RhoA. The results support the hypothesis that the extracellular environment can trigger preferential activation of members of the Rho GTPase family, with demonstrable morphological consequences for cerebral cortical astrocytes. Dove Medical Press 2012 2012-07-20 /pmc/articles/PMC3418172/ /pubmed/22915841 http://dx.doi.org/10.2147/IJN.S32681 Text en © 2012 Tiryaki et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Tiryaki, Volkan Mujdat Ayres, Virginia M Khan, Adeel A Ahmed, Ijaz Shreiber, David I Meiners, Sally Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes |
title | Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes |
title_full | Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes |
title_fullStr | Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes |
title_full_unstemmed | Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes |
title_short | Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes |
title_sort | nanofibrillar scaffolds induce preferential activation of rho gtpases in cerebral cortical astrocytes |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418172/ https://www.ncbi.nlm.nih.gov/pubmed/22915841 http://dx.doi.org/10.2147/IJN.S32681 |
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