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Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography

BACKGROUND: In vivo high-resolution micro-computed tomography allows for longitudinal image-based measurements in animal models of lung disease. The combination of repetitive high resolution imaging with fully automated quantitative image analysis in mouse models of lung fibrosis lung benefits precl...

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Autores principales: De Langhe, Ellen, Vande Velde, Greetje, Hostens, Jeroen, Himmelreich, Uwe, Nemery, Benoit, Luyten, Frank P., Vanoirbeek, Jeroen, Lories, Rik J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418271/
https://www.ncbi.nlm.nih.gov/pubmed/22912805
http://dx.doi.org/10.1371/journal.pone.0043123
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author De Langhe, Ellen
Vande Velde, Greetje
Hostens, Jeroen
Himmelreich, Uwe
Nemery, Benoit
Luyten, Frank P.
Vanoirbeek, Jeroen
Lories, Rik J.
author_facet De Langhe, Ellen
Vande Velde, Greetje
Hostens, Jeroen
Himmelreich, Uwe
Nemery, Benoit
Luyten, Frank P.
Vanoirbeek, Jeroen
Lories, Rik J.
author_sort De Langhe, Ellen
collection PubMed
description BACKGROUND: In vivo high-resolution micro-computed tomography allows for longitudinal image-based measurements in animal models of lung disease. The combination of repetitive high resolution imaging with fully automated quantitative image analysis in mouse models of lung fibrosis lung benefits preclinical research. This study aimed to develop and validate such an automated micro-computed tomography analysis algorithm for quantification of aerated lung volume in mice; an indicator of pulmonary fibrosis and emphysema severity. METHODOLOGY: Mice received an intratracheal instillation of bleomycin (n = 8), elastase (0.25U elastase n = 9, 0.5U elastase n = 8) or saline control (n = 6 for fibrosis, n = 5 for emphysema). A subset of mice was scanned without intervention, to evaluate potential radiation-induced toxicity (n = 4). Some bleomycin-instilled mice were treated with imatinib for proof of concept (n = 8). Mice were scanned weekly, until four weeks after induction, when they underwent pulmonary function testing, lung histology and collagen quantification. Aerated lung volumes were calculated with our automated algorithm. PRINCIPAL FINDINGS: Our automated image-based aerated lung volume quantification method is reproducible with low intra-subject variability. Bleomycin-treated mice had significantly lower scan-derived aerated lung volumes, compared to controls. Aerated lung volume correlated with the histopathological fibrosis score and total lung collagen content. Inversely, a dose-dependent increase in lung volume was observed in elastase-treated mice. Serial scanning of individual mice is feasible and visualized dynamic disease progression. No radiation-induced toxicity was observed. Three-dimensional images provided critical topographical information. CONCLUSIONS: We report on a high resolution in vivo micro-computed tomography image analysis algorithm that runs fully automated and allows quantification of aerated lung volume in mice. This method is reproducible with low inherent measurement variability. We show that it is a reliable quantitative tool to investigate experimental lung fibrosis and emphysema in mice. Its non-invasive nature has the unique benefit to allow dynamic 4D evaluation of disease processes and therapeutic interventions.
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spelling pubmed-34182712012-08-21 Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography De Langhe, Ellen Vande Velde, Greetje Hostens, Jeroen Himmelreich, Uwe Nemery, Benoit Luyten, Frank P. Vanoirbeek, Jeroen Lories, Rik J. PLoS One Research Article BACKGROUND: In vivo high-resolution micro-computed tomography allows for longitudinal image-based measurements in animal models of lung disease. The combination of repetitive high resolution imaging with fully automated quantitative image analysis in mouse models of lung fibrosis lung benefits preclinical research. This study aimed to develop and validate such an automated micro-computed tomography analysis algorithm for quantification of aerated lung volume in mice; an indicator of pulmonary fibrosis and emphysema severity. METHODOLOGY: Mice received an intratracheal instillation of bleomycin (n = 8), elastase (0.25U elastase n = 9, 0.5U elastase n = 8) or saline control (n = 6 for fibrosis, n = 5 for emphysema). A subset of mice was scanned without intervention, to evaluate potential radiation-induced toxicity (n = 4). Some bleomycin-instilled mice were treated with imatinib for proof of concept (n = 8). Mice were scanned weekly, until four weeks after induction, when they underwent pulmonary function testing, lung histology and collagen quantification. Aerated lung volumes were calculated with our automated algorithm. PRINCIPAL FINDINGS: Our automated image-based aerated lung volume quantification method is reproducible with low intra-subject variability. Bleomycin-treated mice had significantly lower scan-derived aerated lung volumes, compared to controls. Aerated lung volume correlated with the histopathological fibrosis score and total lung collagen content. Inversely, a dose-dependent increase in lung volume was observed in elastase-treated mice. Serial scanning of individual mice is feasible and visualized dynamic disease progression. No radiation-induced toxicity was observed. Three-dimensional images provided critical topographical information. CONCLUSIONS: We report on a high resolution in vivo micro-computed tomography image analysis algorithm that runs fully automated and allows quantification of aerated lung volume in mice. This method is reproducible with low inherent measurement variability. We show that it is a reliable quantitative tool to investigate experimental lung fibrosis and emphysema in mice. Its non-invasive nature has the unique benefit to allow dynamic 4D evaluation of disease processes and therapeutic interventions. Public Library of Science 2012-08-13 /pmc/articles/PMC3418271/ /pubmed/22912805 http://dx.doi.org/10.1371/journal.pone.0043123 Text en © 2012 De Langhe et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
De Langhe, Ellen
Vande Velde, Greetje
Hostens, Jeroen
Himmelreich, Uwe
Nemery, Benoit
Luyten, Frank P.
Vanoirbeek, Jeroen
Lories, Rik J.
Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
title Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
title_full Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
title_fullStr Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
title_full_unstemmed Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
title_short Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
title_sort quantification of lung fibrosis and emphysema in mice using automated micro-computed tomography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418271/
https://www.ncbi.nlm.nih.gov/pubmed/22912805
http://dx.doi.org/10.1371/journal.pone.0043123
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