Cargando…
Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species
Transmission of avian influenza viruses (AIV) between different avian species may require genome mutations that allow efficient virus replication in a new species and could increase virulence. To study the role of domestic poultry in the evolution of AIV we compared replication of low pathogenic (LP...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418272/ https://www.ncbi.nlm.nih.gov/pubmed/22912693 http://dx.doi.org/10.1371/journal.pone.0042260 |
_version_ | 1782240623875588096 |
---|---|
author | Petersen, Henning Matrosovich, Mikhail Pleschka, Stephan Rautenschlein, Silke |
author_facet | Petersen, Henning Matrosovich, Mikhail Pleschka, Stephan Rautenschlein, Silke |
author_sort | Petersen, Henning |
collection | PubMed |
description | Transmission of avian influenza viruses (AIV) between different avian species may require genome mutations that allow efficient virus replication in a new species and could increase virulence. To study the role of domestic poultry in the evolution of AIV we compared replication of low pathogenic (LP) AIV of subtypes H9N2, H7N7 and H6N8 in tracheal organ cultures (TOC) and primary embryo fibroblast cultures of chicken, turkey, Pekin duck and homing pigeon. Virus strain-dependent and avian species-related differences between LPAIV were observed in growth kinetics and induction of ciliostasis in TOC. In particular, our data demonstrate high susceptibility to LPAIV of turkey TOC contrasted with low susceptibility of homing pigeon TOC. Serial virus passages in the cells of heterologous host species resulted in adaptive mutations in the AIV genome, especially in the receptor-binding site and protease cleavage site of the hemagglutinin. Our data highlight differences in susceptibility of different birds to AIV viruses and emphasizes potential role of poultry in the emergence of new virus variants. |
format | Online Article Text |
id | pubmed-3418272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34182722012-08-21 Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species Petersen, Henning Matrosovich, Mikhail Pleschka, Stephan Rautenschlein, Silke PLoS One Research Article Transmission of avian influenza viruses (AIV) between different avian species may require genome mutations that allow efficient virus replication in a new species and could increase virulence. To study the role of domestic poultry in the evolution of AIV we compared replication of low pathogenic (LP) AIV of subtypes H9N2, H7N7 and H6N8 in tracheal organ cultures (TOC) and primary embryo fibroblast cultures of chicken, turkey, Pekin duck and homing pigeon. Virus strain-dependent and avian species-related differences between LPAIV were observed in growth kinetics and induction of ciliostasis in TOC. In particular, our data demonstrate high susceptibility to LPAIV of turkey TOC contrasted with low susceptibility of homing pigeon TOC. Serial virus passages in the cells of heterologous host species resulted in adaptive mutations in the AIV genome, especially in the receptor-binding site and protease cleavage site of the hemagglutinin. Our data highlight differences in susceptibility of different birds to AIV viruses and emphasizes potential role of poultry in the emergence of new virus variants. Public Library of Science 2012-08-13 /pmc/articles/PMC3418272/ /pubmed/22912693 http://dx.doi.org/10.1371/journal.pone.0042260 Text en © 2012 Petersen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Petersen, Henning Matrosovich, Mikhail Pleschka, Stephan Rautenschlein, Silke Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species |
title | Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species |
title_full | Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species |
title_fullStr | Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species |
title_full_unstemmed | Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species |
title_short | Replication and Adaptive Mutations of Low Pathogenic Avian Influenza Viruses in Tracheal Organ Cultures of Different Avian Species |
title_sort | replication and adaptive mutations of low pathogenic avian influenza viruses in tracheal organ cultures of different avian species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418272/ https://www.ncbi.nlm.nih.gov/pubmed/22912693 http://dx.doi.org/10.1371/journal.pone.0042260 |
work_keys_str_mv | AT petersenhenning replicationandadaptivemutationsoflowpathogenicavianinfluenzavirusesintrachealorganculturesofdifferentavianspecies AT matrosovichmikhail replicationandadaptivemutationsoflowpathogenicavianinfluenzavirusesintrachealorganculturesofdifferentavianspecies AT pleschkastephan replicationandadaptivemutationsoflowpathogenicavianinfluenzavirusesintrachealorganculturesofdifferentavianspecies AT rautenschleinsilke replicationandadaptivemutationsoflowpathogenicavianinfluenzavirusesintrachealorganculturesofdifferentavianspecies |