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Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats

Traumatic spinal cord injury (SCI) results in direct physical damage and the generation of local factors contributing to secondary pathogenesis. Untargeted metabolomic profiling was used to uncover metabolic changes and to identify relationships between metabolites and neurobehavioral functions in t...

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Autores principales: Fujieda, Yusuke, Ueno, Shinya, Ogino, Ryoko, Kuroda, Mariko, Jönsson, Thomas J., Guo, Lining, Bamba, Takeshi, Fukusaki, Eiichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418274/
https://www.ncbi.nlm.nih.gov/pubmed/22912814
http://dx.doi.org/10.1371/journal.pone.0043152
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author Fujieda, Yusuke
Ueno, Shinya
Ogino, Ryoko
Kuroda, Mariko
Jönsson, Thomas J.
Guo, Lining
Bamba, Takeshi
Fukusaki, Eiichiro
author_facet Fujieda, Yusuke
Ueno, Shinya
Ogino, Ryoko
Kuroda, Mariko
Jönsson, Thomas J.
Guo, Lining
Bamba, Takeshi
Fukusaki, Eiichiro
author_sort Fujieda, Yusuke
collection PubMed
description Traumatic spinal cord injury (SCI) results in direct physical damage and the generation of local factors contributing to secondary pathogenesis. Untargeted metabolomic profiling was used to uncover metabolic changes and to identify relationships between metabolites and neurobehavioral functions in the spinal cord after injury in rats. In the early metabolic phase, neuronal signaling, stress, and inflammation-associated metabolites were strongly altered. A dynamic inflammatory response consisting of elevated levels of prostaglandin E2 and palmitoyl ethanolamide as well as pro- and anti-inflammatory polyunsaturated fatty acids was observed. N-acetyl-aspartyl-glutamate (NAAG) and N-acetyl-aspartate (NAA) were significantly decreased possibly reflecting neuronal cell death. A second metabolic phase was also seen, consistent with membrane remodeling and antioxidant defense response. These metabolomic changes were consistent with the pathology and progression of SCI. Several metabolites, including NAA, NAAG, and the ω-3 fatty acids docosapentaenoate and docosahexaenoate correlated greatly with the established Basso, Beattie and Bresnahan locomotive score (BBB score). Our findings suggest the possibility of a biochemical basis for BBB score and illustrate that metabolites may correlate with neurobehavior. In particular the NAA level in the spinal cord might provide a meaningful biomarker that could help to determine the degree of injury severity and prognosticate neurologic recovery.
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spelling pubmed-34182742012-08-21 Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats Fujieda, Yusuke Ueno, Shinya Ogino, Ryoko Kuroda, Mariko Jönsson, Thomas J. Guo, Lining Bamba, Takeshi Fukusaki, Eiichiro PLoS One Research Article Traumatic spinal cord injury (SCI) results in direct physical damage and the generation of local factors contributing to secondary pathogenesis. Untargeted metabolomic profiling was used to uncover metabolic changes and to identify relationships between metabolites and neurobehavioral functions in the spinal cord after injury in rats. In the early metabolic phase, neuronal signaling, stress, and inflammation-associated metabolites were strongly altered. A dynamic inflammatory response consisting of elevated levels of prostaglandin E2 and palmitoyl ethanolamide as well as pro- and anti-inflammatory polyunsaturated fatty acids was observed. N-acetyl-aspartyl-glutamate (NAAG) and N-acetyl-aspartate (NAA) were significantly decreased possibly reflecting neuronal cell death. A second metabolic phase was also seen, consistent with membrane remodeling and antioxidant defense response. These metabolomic changes were consistent with the pathology and progression of SCI. Several metabolites, including NAA, NAAG, and the ω-3 fatty acids docosapentaenoate and docosahexaenoate correlated greatly with the established Basso, Beattie and Bresnahan locomotive score (BBB score). Our findings suggest the possibility of a biochemical basis for BBB score and illustrate that metabolites may correlate with neurobehavior. In particular the NAA level in the spinal cord might provide a meaningful biomarker that could help to determine the degree of injury severity and prognosticate neurologic recovery. Public Library of Science 2012-08-13 /pmc/articles/PMC3418274/ /pubmed/22912814 http://dx.doi.org/10.1371/journal.pone.0043152 Text en © 2012 Fujieda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fujieda, Yusuke
Ueno, Shinya
Ogino, Ryoko
Kuroda, Mariko
Jönsson, Thomas J.
Guo, Lining
Bamba, Takeshi
Fukusaki, Eiichiro
Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats
title Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats
title_full Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats
title_fullStr Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats
title_full_unstemmed Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats
title_short Metabolite Profiles Correlate Closely with Neurobehavioral Function in Experimental Spinal Cord Injury in Rats
title_sort metabolite profiles correlate closely with neurobehavioral function in experimental spinal cord injury in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418274/
https://www.ncbi.nlm.nih.gov/pubmed/22912814
http://dx.doi.org/10.1371/journal.pone.0043152
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