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The CSC connects three major axonemal complexes involved in dynein regulation

Motile cilia and flagella are highly conserved organelles that play important roles in human health and development. We recently discovered a calmodulin- and spoke-associ­ated complex (CSC) that is required for wild-type motility and for the stable assembly of a subset of radial spokes. Using cryo–e...

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Autores principales: Heuser, Thomas, Dymek, Erin E., Lin, Jianfeng, Smith, Elizabeth F., Nicastro, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418309/
https://www.ncbi.nlm.nih.gov/pubmed/22740634
http://dx.doi.org/10.1091/mbc.E12-05-0357
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author Heuser, Thomas
Dymek, Erin E.
Lin, Jianfeng
Smith, Elizabeth F.
Nicastro, Daniela
author_facet Heuser, Thomas
Dymek, Erin E.
Lin, Jianfeng
Smith, Elizabeth F.
Nicastro, Daniela
author_sort Heuser, Thomas
collection PubMed
description Motile cilia and flagella are highly conserved organelles that play important roles in human health and development. We recently discovered a calmodulin- and spoke-associ­ated complex (CSC) that is required for wild-type motility and for the stable assembly of a subset of radial spokes. Using cryo–electron tomography, we present the first structure-based localization model of the CSC. Chlamydomonas flagella have two full-length radial spokes, RS1 and RS2, and a shorter RS3 homologue, the RS3 stand-in (RS3S). Using newly developed techniques for analyzing samples with structural heterogeneity, we demonstrate that the CSC connects three major axonemal complexes involved in dynein regulation: RS2, the nexin–dynein regulatory complex (N-DRC), and RS3S. These results provide insights into how signals from the radial spokes may be transmitted to the N-DRC and ultimately to the dynein motors. Our results also indicate that although structurally very similar, RS1 and RS2 likely serve different functions in regulating flagellar motility.
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spelling pubmed-34183092012-10-30 The CSC connects three major axonemal complexes involved in dynein regulation Heuser, Thomas Dymek, Erin E. Lin, Jianfeng Smith, Elizabeth F. Nicastro, Daniela Mol Biol Cell Articles Motile cilia and flagella are highly conserved organelles that play important roles in human health and development. We recently discovered a calmodulin- and spoke-associ­ated complex (CSC) that is required for wild-type motility and for the stable assembly of a subset of radial spokes. Using cryo–electron tomography, we present the first structure-based localization model of the CSC. Chlamydomonas flagella have two full-length radial spokes, RS1 and RS2, and a shorter RS3 homologue, the RS3 stand-in (RS3S). Using newly developed techniques for analyzing samples with structural heterogeneity, we demonstrate that the CSC connects three major axonemal complexes involved in dynein regulation: RS2, the nexin–dynein regulatory complex (N-DRC), and RS3S. These results provide insights into how signals from the radial spokes may be transmitted to the N-DRC and ultimately to the dynein motors. Our results also indicate that although structurally very similar, RS1 and RS2 likely serve different functions in regulating flagellar motility. The American Society for Cell Biology 2012-08-15 /pmc/articles/PMC3418309/ /pubmed/22740634 http://dx.doi.org/10.1091/mbc.E12-05-0357 Text en © 2012 Heuser et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Heuser, Thomas
Dymek, Erin E.
Lin, Jianfeng
Smith, Elizabeth F.
Nicastro, Daniela
The CSC connects three major axonemal complexes involved in dynein regulation
title The CSC connects three major axonemal complexes involved in dynein regulation
title_full The CSC connects three major axonemal complexes involved in dynein regulation
title_fullStr The CSC connects three major axonemal complexes involved in dynein regulation
title_full_unstemmed The CSC connects three major axonemal complexes involved in dynein regulation
title_short The CSC connects three major axonemal complexes involved in dynein regulation
title_sort csc connects three major axonemal complexes involved in dynein regulation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418309/
https://www.ncbi.nlm.nih.gov/pubmed/22740634
http://dx.doi.org/10.1091/mbc.E12-05-0357
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