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Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System

A growing body of evidence indicates that the majority of Parkinson's disease (PD) cases are associated with microglia activation with resultant elevation of various inflammatory mediators and neuroinflammation. In this study, we investigated the effects of 2 natural molecules, quercetin and se...

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Autores principales: Bournival, Julie, Plouffe, Marilyn, Renaud, Justine, Provencher, Cindy, Martinoli, Maria-Grazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418684/
https://www.ncbi.nlm.nih.gov/pubmed/22919443
http://dx.doi.org/10.1155/2012/921941
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author Bournival, Julie
Plouffe, Marilyn
Renaud, Justine
Provencher, Cindy
Martinoli, Maria-Grazia
author_facet Bournival, Julie
Plouffe, Marilyn
Renaud, Justine
Provencher, Cindy
Martinoli, Maria-Grazia
author_sort Bournival, Julie
collection PubMed
description A growing body of evidence indicates that the majority of Parkinson's disease (PD) cases are associated with microglia activation with resultant elevation of various inflammatory mediators and neuroinflammation. In this study, we investigated the effects of 2 natural molecules, quercetin and sesamin, on neuroinflammation induced by the Parkinsonian toxin 1-methyl-4-phenylpyridinium (MPP(+)) in a glial-neuronal system. We first established that quercetin and sesamin defend microglial cells against MPP(+)-induced increases in the mRNA or protein levels of 3 pro-inflammatory cytokines (interleukin-6, IL-1β and tumor necrosis factor-alpha), as revealed by real time-quantitative polymerase chain reaction and enzyme-linked immunoabsorbent assay, respectively. Quercetin and sesamin also decrease MPP(+)-induced oxidative stress in microglial cells by reducing inducible nitric oxide synthase protein expression as well as mitochondrial superoxide radicals. We then measured neuronal cell death and apoptosis after MPP(+) activation of microglia, in a microglial (N9)-neuronal (PC12) coculture system. Our results revealed that quercetin and sesamin rescued neuronal PC12 cells from apoptotic death induced by MPP(+) activation of microglial cells. Altogether, our data demonstrate that the phytoestrogen quercetin and the lignan sesamin diminish MPP(+)-evoked microglial activation and suggest that both these molecules may be regarded as potent, natural, anti-inflammatory compounds.
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spelling pubmed-34186842012-08-23 Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System Bournival, Julie Plouffe, Marilyn Renaud, Justine Provencher, Cindy Martinoli, Maria-Grazia Oxid Med Cell Longev Research Article A growing body of evidence indicates that the majority of Parkinson's disease (PD) cases are associated with microglia activation with resultant elevation of various inflammatory mediators and neuroinflammation. In this study, we investigated the effects of 2 natural molecules, quercetin and sesamin, on neuroinflammation induced by the Parkinsonian toxin 1-methyl-4-phenylpyridinium (MPP(+)) in a glial-neuronal system. We first established that quercetin and sesamin defend microglial cells against MPP(+)-induced increases in the mRNA or protein levels of 3 pro-inflammatory cytokines (interleukin-6, IL-1β and tumor necrosis factor-alpha), as revealed by real time-quantitative polymerase chain reaction and enzyme-linked immunoabsorbent assay, respectively. Quercetin and sesamin also decrease MPP(+)-induced oxidative stress in microglial cells by reducing inducible nitric oxide synthase protein expression as well as mitochondrial superoxide radicals. We then measured neuronal cell death and apoptosis after MPP(+) activation of microglia, in a microglial (N9)-neuronal (PC12) coculture system. Our results revealed that quercetin and sesamin rescued neuronal PC12 cells from apoptotic death induced by MPP(+) activation of microglial cells. Altogether, our data demonstrate that the phytoestrogen quercetin and the lignan sesamin diminish MPP(+)-evoked microglial activation and suggest that both these molecules may be regarded as potent, natural, anti-inflammatory compounds. Hindawi Publishing Corporation 2012 2012-07-03 /pmc/articles/PMC3418684/ /pubmed/22919443 http://dx.doi.org/10.1155/2012/921941 Text en Copyright © 2012 Julie Bournival et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bournival, Julie
Plouffe, Marilyn
Renaud, Justine
Provencher, Cindy
Martinoli, Maria-Grazia
Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
title Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
title_full Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
title_fullStr Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
title_full_unstemmed Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
title_short Quercetin and Sesamin Protect Dopaminergic Cells from MPP(+)-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System
title_sort quercetin and sesamin protect dopaminergic cells from mpp(+)-induced neuroinflammation in a microglial (n9)-neuronal (pc12) coculture system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418684/
https://www.ncbi.nlm.nih.gov/pubmed/22919443
http://dx.doi.org/10.1155/2012/921941
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