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Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals
Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep t...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418925/ https://www.ncbi.nlm.nih.gov/pubmed/22896771 http://dx.doi.org/10.7150/thno.3459 |
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author | Law, Wing-Cheung Mahajan, Supriya D. Kopwitthaya, Atcha Reynolds, Jessica L. Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Schwartz, Stanley A. Yong, Ken-Tye Prasad, Paras N. |
author_facet | Law, Wing-Cheung Mahajan, Supriya D. Kopwitthaya, Atcha Reynolds, Jessica L. Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Schwartz, Stanley A. Yong, Ken-Tye Prasad, Paras N. |
author_sort | Law, Wing-Cheung |
collection | PubMed |
description | Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep track on the therapeutic agent such as the pharmacokinetics and biodistribution. In this report, we have developed quantum rod (QR) based formulations for the delivery of small interfering RNAs (siRNAs) to human neuronal cells. PEGlyated QRs with different surface functional groups (amine and maleimide) were designed for selectively down-regulating the dopaminergic signaling pathway which is associated with the drug abuse behavior. We have demonstrated that the DARPP-32 siRNAs were successfully delivered to dopaminergic neuronal (DAN) cells which led to drastic knockdown of specific gene expression by both the electrostatic and covalent bond conjugation regimes. The PEGlyated surface offered high biocompatibilities and negligible cytotoxicities to the QR formulations that may facilitate the in vivo applications of these nanoparticles. |
format | Online Article Text |
id | pubmed-3418925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-34189252012-08-15 Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals Law, Wing-Cheung Mahajan, Supriya D. Kopwitthaya, Atcha Reynolds, Jessica L. Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Schwartz, Stanley A. Yong, Ken-Tye Prasad, Paras N. Theranostics Research Paper Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep track on the therapeutic agent such as the pharmacokinetics and biodistribution. In this report, we have developed quantum rod (QR) based formulations for the delivery of small interfering RNAs (siRNAs) to human neuronal cells. PEGlyated QRs with different surface functional groups (amine and maleimide) were designed for selectively down-regulating the dopaminergic signaling pathway which is associated with the drug abuse behavior. We have demonstrated that the DARPP-32 siRNAs were successfully delivered to dopaminergic neuronal (DAN) cells which led to drastic knockdown of specific gene expression by both the electrostatic and covalent bond conjugation regimes. The PEGlyated surface offered high biocompatibilities and negligible cytotoxicities to the QR formulations that may facilitate the in vivo applications of these nanoparticles. Ivyspring International Publisher 2012-07-27 /pmc/articles/PMC3418925/ /pubmed/22896771 http://dx.doi.org/10.7150/thno.3459 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Law, Wing-Cheung Mahajan, Supriya D. Kopwitthaya, Atcha Reynolds, Jessica L. Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Schwartz, Stanley A. Yong, Ken-Tye Prasad, Paras N. Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals |
title | Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals |
title_full | Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals |
title_fullStr | Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals |
title_full_unstemmed | Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals |
title_short | Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals |
title_sort | gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418925/ https://www.ncbi.nlm.nih.gov/pubmed/22896771 http://dx.doi.org/10.7150/thno.3459 |
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