Caged intracellular NMDA receptor blockers for the study of subcellular ion channel function

We have previously synthesized a caged form of the use-dependent N-methyl-D-aspartate (NMDA) receptor ion channel blocker MK801 and used intracellular photolysis of this compound to demonstrate the subcellular location of NMDA receptor ion channels involved in synaptic plasticity. Here, we discuss c...

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Detalles Bibliográficos
Autores principales: Reeve, James E., Kohl, Michael M., Rodríguez-Moreno, Antonio, Paulsen, Ole, Anderson, Harry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419105/
https://www.ncbi.nlm.nih.gov/pubmed/22896783
http://dx.doi.org/10.4161/cib.19400
Descripción
Sumario:We have previously synthesized a caged form of the use-dependent N-methyl-D-aspartate (NMDA) receptor ion channel blocker MK801 and used intracellular photolysis of this compound to demonstrate the subcellular location of NMDA receptor ion channels involved in synaptic plasticity. Here, we discuss considerations regarding the choice of caging molecule, synthesis and the potential uses for caged ion channel blockers in neurophysiology.

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