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Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates
An effective anti-human immunodeficiency virus-1 (HIV-1) microbicide should exert its action in the absence of causing aberrant activation of topical immunity that will increase the risk of HIV acquisition. In the present study, we demonstrated that the vaginal application of cellulose sulfate (CS)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419165/ https://www.ncbi.nlm.nih.gov/pubmed/22905236 http://dx.doi.org/10.1371/journal.pone.0043211 |
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author | Li, Liangzhu Ben, Yinyin Zhu, Zhaoqin Li, Weihua Xu, Jianqing Zhang, Xiaoyan |
author_facet | Li, Liangzhu Ben, Yinyin Zhu, Zhaoqin Li, Weihua Xu, Jianqing Zhang, Xiaoyan |
author_sort | Li, Liangzhu |
collection | PubMed |
description | An effective anti-human immunodeficiency virus-1 (HIV-1) microbicide should exert its action in the absence of causing aberrant activation of topical immunity that will increase the risk of HIV acquisition. In the present study, we demonstrated that the vaginal application of cellulose sulfate (CS) gel induced topical mucosal inflammatory responses; the addition of minocycline to CS gel could significantly attenuate the inflammation in a mice model. The combined gel of CS plus minocycline not only reduced the production of inflammatory cytokines in cervicovaginal lavages (CVLs), also down-regulated the activation of CD4+ T cells and the recruitment of other immune cells including HIV target cells into vaginal tissues. Furthermore, an In vitro HIV-1 pseudovirus infection inhibition assay showed that the combined gel decreased the infection efficacy of different subtypes of HIV-1 pseudoviruses compared with that of CS gel alone. These results implicate that minocycline could be integrated into microbicide formulation to suppress the aberrant activation of topical mucosal immunity and enhance the safety profile during the application of microbicides. |
format | Online Article Text |
id | pubmed-3419165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34191652012-08-19 Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates Li, Liangzhu Ben, Yinyin Zhu, Zhaoqin Li, Weihua Xu, Jianqing Zhang, Xiaoyan PLoS One Research Article An effective anti-human immunodeficiency virus-1 (HIV-1) microbicide should exert its action in the absence of causing aberrant activation of topical immunity that will increase the risk of HIV acquisition. In the present study, we demonstrated that the vaginal application of cellulose sulfate (CS) gel induced topical mucosal inflammatory responses; the addition of minocycline to CS gel could significantly attenuate the inflammation in a mice model. The combined gel of CS plus minocycline not only reduced the production of inflammatory cytokines in cervicovaginal lavages (CVLs), also down-regulated the activation of CD4+ T cells and the recruitment of other immune cells including HIV target cells into vaginal tissues. Furthermore, an In vitro HIV-1 pseudovirus infection inhibition assay showed that the combined gel decreased the infection efficacy of different subtypes of HIV-1 pseudoviruses compared with that of CS gel alone. These results implicate that minocycline could be integrated into microbicide formulation to suppress the aberrant activation of topical mucosal immunity and enhance the safety profile during the application of microbicides. Public Library of Science 2012-08-14 /pmc/articles/PMC3419165/ /pubmed/22905236 http://dx.doi.org/10.1371/journal.pone.0043211 Text en © 2012 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Liangzhu Ben, Yinyin Zhu, Zhaoqin Li, Weihua Xu, Jianqing Zhang, Xiaoyan Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates |
title | Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates |
title_full | Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates |
title_fullStr | Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates |
title_full_unstemmed | Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates |
title_short | Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates |
title_sort | minocycline down-regulates topical mucosal inflammation during the application of microbicide candidates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419165/ https://www.ncbi.nlm.nih.gov/pubmed/22905236 http://dx.doi.org/10.1371/journal.pone.0043211 |
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