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The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed bot...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419242/ https://www.ncbi.nlm.nih.gov/pubmed/22916179 http://dx.doi.org/10.1371/journal.pone.0042914 |
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author | Neumetzler, Lutz Humphrey, Tania Lumba, Shelley Snyder, Stephen Yeats, Trevor H. Usadel, Björn Vasilevski, Aleksandar Patel, Jignasha Rose, Jocelyn K. C. Persson, Staffan Bonetta, Dario |
author_facet | Neumetzler, Lutz Humphrey, Tania Lumba, Shelley Snyder, Stephen Yeats, Trevor H. Usadel, Björn Vasilevski, Aleksandar Patel, Jignasha Rose, Jocelyn K. C. Persson, Staffan Bonetta, Dario |
author_sort | Neumetzler, Lutz |
collection | PubMed |
description | Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion. |
format | Online Article Text |
id | pubmed-3419242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34192422012-08-22 The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis Neumetzler, Lutz Humphrey, Tania Lumba, Shelley Snyder, Stephen Yeats, Trevor H. Usadel, Björn Vasilevski, Aleksandar Patel, Jignasha Rose, Jocelyn K. C. Persson, Staffan Bonetta, Dario PLoS One Research Article Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion. Public Library of Science 2012-08-14 /pmc/articles/PMC3419242/ /pubmed/22916179 http://dx.doi.org/10.1371/journal.pone.0042914 Text en © 2012 Neumetzler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Neumetzler, Lutz Humphrey, Tania Lumba, Shelley Snyder, Stephen Yeats, Trevor H. Usadel, Björn Vasilevski, Aleksandar Patel, Jignasha Rose, Jocelyn K. C. Persson, Staffan Bonetta, Dario The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis |
title | The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis |
title_full | The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis |
title_fullStr | The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis |
title_full_unstemmed | The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis |
title_short | The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis |
title_sort | friable1 gene product affects cell adhesion in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419242/ https://www.ncbi.nlm.nih.gov/pubmed/22916179 http://dx.doi.org/10.1371/journal.pone.0042914 |
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