Cargando…

The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis

Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed bot...

Descripción completa

Detalles Bibliográficos
Autores principales: Neumetzler, Lutz, Humphrey, Tania, Lumba, Shelley, Snyder, Stephen, Yeats, Trevor H., Usadel, Björn, Vasilevski, Aleksandar, Patel, Jignasha, Rose, Jocelyn K. C., Persson, Staffan, Bonetta, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419242/
https://www.ncbi.nlm.nih.gov/pubmed/22916179
http://dx.doi.org/10.1371/journal.pone.0042914
_version_ 1782240711302709248
author Neumetzler, Lutz
Humphrey, Tania
Lumba, Shelley
Snyder, Stephen
Yeats, Trevor H.
Usadel, Björn
Vasilevski, Aleksandar
Patel, Jignasha
Rose, Jocelyn K. C.
Persson, Staffan
Bonetta, Dario
author_facet Neumetzler, Lutz
Humphrey, Tania
Lumba, Shelley
Snyder, Stephen
Yeats, Trevor H.
Usadel, Björn
Vasilevski, Aleksandar
Patel, Jignasha
Rose, Jocelyn K. C.
Persson, Staffan
Bonetta, Dario
author_sort Neumetzler, Lutz
collection PubMed
description Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion.
format Online
Article
Text
id pubmed-3419242
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34192422012-08-22 The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis Neumetzler, Lutz Humphrey, Tania Lumba, Shelley Snyder, Stephen Yeats, Trevor H. Usadel, Björn Vasilevski, Aleksandar Patel, Jignasha Rose, Jocelyn K. C. Persson, Staffan Bonetta, Dario PLoS One Research Article Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion. Public Library of Science 2012-08-14 /pmc/articles/PMC3419242/ /pubmed/22916179 http://dx.doi.org/10.1371/journal.pone.0042914 Text en © 2012 Neumetzler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Neumetzler, Lutz
Humphrey, Tania
Lumba, Shelley
Snyder, Stephen
Yeats, Trevor H.
Usadel, Björn
Vasilevski, Aleksandar
Patel, Jignasha
Rose, Jocelyn K. C.
Persson, Staffan
Bonetta, Dario
The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
title The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
title_full The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
title_fullStr The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
title_full_unstemmed The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
title_short The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis
title_sort friable1 gene product affects cell adhesion in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419242/
https://www.ncbi.nlm.nih.gov/pubmed/22916179
http://dx.doi.org/10.1371/journal.pone.0042914
work_keys_str_mv AT neumetzlerlutz thefriable1geneproductaffectscelladhesioninarabidopsis
AT humphreytania thefriable1geneproductaffectscelladhesioninarabidopsis
AT lumbashelley thefriable1geneproductaffectscelladhesioninarabidopsis
AT snyderstephen thefriable1geneproductaffectscelladhesioninarabidopsis
AT yeatstrevorh thefriable1geneproductaffectscelladhesioninarabidopsis
AT usadelbjorn thefriable1geneproductaffectscelladhesioninarabidopsis
AT vasilevskialeksandar thefriable1geneproductaffectscelladhesioninarabidopsis
AT pateljignasha thefriable1geneproductaffectscelladhesioninarabidopsis
AT rosejocelynkc thefriable1geneproductaffectscelladhesioninarabidopsis
AT perssonstaffan thefriable1geneproductaffectscelladhesioninarabidopsis
AT bonettadario thefriable1geneproductaffectscelladhesioninarabidopsis
AT neumetzlerlutz friable1geneproductaffectscelladhesioninarabidopsis
AT humphreytania friable1geneproductaffectscelladhesioninarabidopsis
AT lumbashelley friable1geneproductaffectscelladhesioninarabidopsis
AT snyderstephen friable1geneproductaffectscelladhesioninarabidopsis
AT yeatstrevorh friable1geneproductaffectscelladhesioninarabidopsis
AT usadelbjorn friable1geneproductaffectscelladhesioninarabidopsis
AT vasilevskialeksandar friable1geneproductaffectscelladhesioninarabidopsis
AT pateljignasha friable1geneproductaffectscelladhesioninarabidopsis
AT rosejocelynkc friable1geneproductaffectscelladhesioninarabidopsis
AT perssonstaffan friable1geneproductaffectscelladhesioninarabidopsis
AT bonettadario friable1geneproductaffectscelladhesioninarabidopsis