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Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens

BACKGROUND: Environmental surfaces play an important role in transmission of healthcare-associated pathogens. There is a need for new disinfection methods that are effective against Clostridium difficile spores, but also safe and rapid. The Sterilray™ Disinfection Wand device is a hand-held room dec...

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Autores principales: Nerandzic, Michelle M, Cadnum, Jennifer L, Eckart, Kevin E, Donskey, Curtis J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419611/
https://www.ncbi.nlm.nih.gov/pubmed/22591268
http://dx.doi.org/10.1186/1471-2334-12-120
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author Nerandzic, Michelle M
Cadnum, Jennifer L
Eckart, Kevin E
Donskey, Curtis J
author_facet Nerandzic, Michelle M
Cadnum, Jennifer L
Eckart, Kevin E
Donskey, Curtis J
author_sort Nerandzic, Michelle M
collection PubMed
description BACKGROUND: Environmental surfaces play an important role in transmission of healthcare-associated pathogens. There is a need for new disinfection methods that are effective against Clostridium difficile spores, but also safe and rapid. The Sterilray™ Disinfection Wand device is a hand-held room decontamination technology that utilizes far-ultraviolet radiation (185-230 nm) to kill pathogens. METHODS: We examined the efficacy of disinfection using the Sterilray device in the laboratory, in rooms of hospitalized patients, and on surfaces outside of patient rooms (i.e. keyboards and portable medical equipment). Cultures for C. difficile, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus (VRE) were collected from commonly-touched surfaces before and after use of the Sterilray device. RESULTS: On inoculated surfaces in the laboratory, application of the Sterilray device at a radiant dose of 100 mJ/cm(2) for ~ 5 seconds consistently reduced recovery of C. difficile spores by 4.4 CFU log(10), MRSA by 5.4 log(10)CFU and of VRE by 6.9 log(10)CFU. A >3 log(10) reduction of MRSA and VRE was achieved in ~2 seconds at a lower radiant dose, but killing of C. difficile spores was significantly reduced. On keyboards and portable medical equipment that were inoculated with C. difficile spores, application of the Sterilray device at a radiant dose of 100 mJ/cm(2) for ~ 5 seconds reduced contamination by 3.2 log(10)CFU. However, the presence of organic material reduced the lethal effect of the far-UV radiation. In hospital rooms that were not pre-cleaned, disinfection with the Sterilray device significantly reduced the frequency of positive C. difficile and MRSA cultures (P =0.007). CONCLUSIONS: The Sterilray™ Disinfection Wand is a novel environmental disinfection technology that rapidly kills C. difficile spores and other healthcare-associated pathogens on surfaces. However, the presence of organic matter reduces the efficacy of far-UV radiation, possibly explaining the more modest results observed on surfaces in hospital rooms that were not pre-cleaned.
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spelling pubmed-34196112012-08-16 Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens Nerandzic, Michelle M Cadnum, Jennifer L Eckart, Kevin E Donskey, Curtis J BMC Infect Dis Research Article BACKGROUND: Environmental surfaces play an important role in transmission of healthcare-associated pathogens. There is a need for new disinfection methods that are effective against Clostridium difficile spores, but also safe and rapid. The Sterilray™ Disinfection Wand device is a hand-held room decontamination technology that utilizes far-ultraviolet radiation (185-230 nm) to kill pathogens. METHODS: We examined the efficacy of disinfection using the Sterilray device in the laboratory, in rooms of hospitalized patients, and on surfaces outside of patient rooms (i.e. keyboards and portable medical equipment). Cultures for C. difficile, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus (VRE) were collected from commonly-touched surfaces before and after use of the Sterilray device. RESULTS: On inoculated surfaces in the laboratory, application of the Sterilray device at a radiant dose of 100 mJ/cm(2) for ~ 5 seconds consistently reduced recovery of C. difficile spores by 4.4 CFU log(10), MRSA by 5.4 log(10)CFU and of VRE by 6.9 log(10)CFU. A >3 log(10) reduction of MRSA and VRE was achieved in ~2 seconds at a lower radiant dose, but killing of C. difficile spores was significantly reduced. On keyboards and portable medical equipment that were inoculated with C. difficile spores, application of the Sterilray device at a radiant dose of 100 mJ/cm(2) for ~ 5 seconds reduced contamination by 3.2 log(10)CFU. However, the presence of organic material reduced the lethal effect of the far-UV radiation. In hospital rooms that were not pre-cleaned, disinfection with the Sterilray device significantly reduced the frequency of positive C. difficile and MRSA cultures (P =0.007). CONCLUSIONS: The Sterilray™ Disinfection Wand is a novel environmental disinfection technology that rapidly kills C. difficile spores and other healthcare-associated pathogens on surfaces. However, the presence of organic matter reduces the efficacy of far-UV radiation, possibly explaining the more modest results observed on surfaces in hospital rooms that were not pre-cleaned. BioMed Central 2012-05-16 /pmc/articles/PMC3419611/ /pubmed/22591268 http://dx.doi.org/10.1186/1471-2334-12-120 Text en Copyright ©2012 Nerandzic et al.; licensee BioMed Central Ltd http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nerandzic, Michelle M
Cadnum, Jennifer L
Eckart, Kevin E
Donskey, Curtis J
Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens
title Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens
title_full Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens
title_fullStr Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens
title_full_unstemmed Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens
title_short Evaluation of a hand-held far-ultraviolet radiation device for decontamination of Clostridium difficile and other healthcare-associated pathogens
title_sort evaluation of a hand-held far-ultraviolet radiation device for decontamination of clostridium difficile and other healthcare-associated pathogens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419611/
https://www.ncbi.nlm.nih.gov/pubmed/22591268
http://dx.doi.org/10.1186/1471-2334-12-120
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