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Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome

Obesity has been linked to the human gut microbiota; however, the contribution of gut bacterial species to the obese phenotype remains controversial because of conflicting results from studies in different populations. To explore the possible dysbiosis of gut microbiota in obesity and its metabolic...

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Autores principales: Zupancic, Margaret L., Cantarel, Brandi L., Liu, Zhenqiu, Drabek, Elliott F., Ryan, Kathleen A., Cirimotich, Shana, Jones, Cheron, Knight, Rob, Walters, William A., Knights, Daniel, Mongodin, Emmanuel F., Horenstein, Richard B., Mitchell, Braxton D., Steinle, Nanette, Snitker, Soren, Shuldiner, Alan R., Fraser, Claire M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419686/
https://www.ncbi.nlm.nih.gov/pubmed/22905200
http://dx.doi.org/10.1371/journal.pone.0043052
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author Zupancic, Margaret L.
Cantarel, Brandi L.
Liu, Zhenqiu
Drabek, Elliott F.
Ryan, Kathleen A.
Cirimotich, Shana
Jones, Cheron
Knight, Rob
Walters, William A.
Knights, Daniel
Mongodin, Emmanuel F.
Horenstein, Richard B.
Mitchell, Braxton D.
Steinle, Nanette
Snitker, Soren
Shuldiner, Alan R.
Fraser, Claire M.
author_facet Zupancic, Margaret L.
Cantarel, Brandi L.
Liu, Zhenqiu
Drabek, Elliott F.
Ryan, Kathleen A.
Cirimotich, Shana
Jones, Cheron
Knight, Rob
Walters, William A.
Knights, Daniel
Mongodin, Emmanuel F.
Horenstein, Richard B.
Mitchell, Braxton D.
Steinle, Nanette
Snitker, Soren
Shuldiner, Alan R.
Fraser, Claire M.
author_sort Zupancic, Margaret L.
collection PubMed
description Obesity has been linked to the human gut microbiota; however, the contribution of gut bacterial species to the obese phenotype remains controversial because of conflicting results from studies in different populations. To explore the possible dysbiosis of gut microbiota in obesity and its metabolic complications, we studied men and women over a range of body mass indices from the Old Order Amish sect, a culturally homogeneous Caucasian population of Central European ancestry. We characterized the gut microbiota in 310 subjects by deep pyrosequencing of bar-coded PCR amplicons from the V1–V3 region of the 16S rRNA gene. Three communities of interacting bacteria were identified in the gut microbiota, analogous to previously identified gut enterotypes. Neither BMI nor any metabolic syndrome trait was associated with a particular gut community. Network analysis identified twenty-two bacterial species and four OTUs that were either positively or inversely correlated with metabolic syndrome traits, suggesting that certain members of the gut microbiota may play a role in these metabolic derangements.
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spelling pubmed-34196862012-08-17 Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome Zupancic, Margaret L. Cantarel, Brandi L. Liu, Zhenqiu Drabek, Elliott F. Ryan, Kathleen A. Cirimotich, Shana Jones, Cheron Knight, Rob Walters, William A. Knights, Daniel Mongodin, Emmanuel F. Horenstein, Richard B. Mitchell, Braxton D. Steinle, Nanette Snitker, Soren Shuldiner, Alan R. Fraser, Claire M. PLoS One Research Article Obesity has been linked to the human gut microbiota; however, the contribution of gut bacterial species to the obese phenotype remains controversial because of conflicting results from studies in different populations. To explore the possible dysbiosis of gut microbiota in obesity and its metabolic complications, we studied men and women over a range of body mass indices from the Old Order Amish sect, a culturally homogeneous Caucasian population of Central European ancestry. We characterized the gut microbiota in 310 subjects by deep pyrosequencing of bar-coded PCR amplicons from the V1–V3 region of the 16S rRNA gene. Three communities of interacting bacteria were identified in the gut microbiota, analogous to previously identified gut enterotypes. Neither BMI nor any metabolic syndrome trait was associated with a particular gut community. Network analysis identified twenty-two bacterial species and four OTUs that were either positively or inversely correlated with metabolic syndrome traits, suggesting that certain members of the gut microbiota may play a role in these metabolic derangements. Public Library of Science 2012-08-15 /pmc/articles/PMC3419686/ /pubmed/22905200 http://dx.doi.org/10.1371/journal.pone.0043052 Text en © 2012 Zupancic et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zupancic, Margaret L.
Cantarel, Brandi L.
Liu, Zhenqiu
Drabek, Elliott F.
Ryan, Kathleen A.
Cirimotich, Shana
Jones, Cheron
Knight, Rob
Walters, William A.
Knights, Daniel
Mongodin, Emmanuel F.
Horenstein, Richard B.
Mitchell, Braxton D.
Steinle, Nanette
Snitker, Soren
Shuldiner, Alan R.
Fraser, Claire M.
Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome
title Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome
title_full Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome
title_fullStr Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome
title_full_unstemmed Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome
title_short Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome
title_sort analysis of the gut microbiota in the old order amish and its relation to the metabolic syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419686/
https://www.ncbi.nlm.nih.gov/pubmed/22905200
http://dx.doi.org/10.1371/journal.pone.0043052
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