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Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis

Infection with Burkholderia pseudomallei causes the disease melioidosis, which often presents as a serious suppurative infection that is typically fatal without intensive treatment and is a significant emerging infectious disease in Southeast Asia. Despite intensive research there is still much that...

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Autores principales: Soffler, Carl, Bosco-Lauth, Angela M., Aboellail, Tawfik A., Marolf, Angela J., Bowen, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419728/
https://www.ncbi.nlm.nih.gov/pubmed/22916225
http://dx.doi.org/10.1371/journal.pone.0043207
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author Soffler, Carl
Bosco-Lauth, Angela M.
Aboellail, Tawfik A.
Marolf, Angela J.
Bowen, Richard A.
author_facet Soffler, Carl
Bosco-Lauth, Angela M.
Aboellail, Tawfik A.
Marolf, Angela J.
Bowen, Richard A.
author_sort Soffler, Carl
collection PubMed
description Infection with Burkholderia pseudomallei causes the disease melioidosis, which often presents as a serious suppurative infection that is typically fatal without intensive treatment and is a significant emerging infectious disease in Southeast Asia. Despite intensive research there is still much that remains unknown about melioidosis pathogenesis. New animal models of melioidosis are needed to examine novel aspects of pathogenesis as well as for the evaluation of novel therapeutics. The objective of the work presented here was to develop a subacute to chronic caprine model of melioidosis and to characterize the progression of disease with respect to clinical presentation, hematology, clinical microbiology, thoracic radiography, and gross and microscopic pathology. Disease was produced in all animals following an intratracheal aerosol of 10(4) CFU delivered, with variable clinical manifestations indicative of subacute and chronic disease. Bronchointerstitial pneumonia was apparent microscopically by day 2 and radiographically and grossly apparent by day 7 post infection (PI). Early lesions of bronchopneumonia soon progressed to more severe bronchointerstitial pneumonia with pyogranuloma formation. Extrapulmonary dissemination appeared to be a function of pyogranuloma invasion of pulmonary vasculature, which peaked around day 7 PI. Histopathology indicated that leukocytoclastic vasculitis was the central step in dissemination of B. pseudomallei from the lungs as well as in the establishment of new lesions. While higher doses of organism in goats can produce acute fatal disease, the dose investigated and resulting disease had many similarities to human melioidosis and may warrant further development to provide a model for the study of both natural and bioterrorism associated disease.
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spelling pubmed-34197282012-08-22 Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis Soffler, Carl Bosco-Lauth, Angela M. Aboellail, Tawfik A. Marolf, Angela J. Bowen, Richard A. PLoS One Research Article Infection with Burkholderia pseudomallei causes the disease melioidosis, which often presents as a serious suppurative infection that is typically fatal without intensive treatment and is a significant emerging infectious disease in Southeast Asia. Despite intensive research there is still much that remains unknown about melioidosis pathogenesis. New animal models of melioidosis are needed to examine novel aspects of pathogenesis as well as for the evaluation of novel therapeutics. The objective of the work presented here was to develop a subacute to chronic caprine model of melioidosis and to characterize the progression of disease with respect to clinical presentation, hematology, clinical microbiology, thoracic radiography, and gross and microscopic pathology. Disease was produced in all animals following an intratracheal aerosol of 10(4) CFU delivered, with variable clinical manifestations indicative of subacute and chronic disease. Bronchointerstitial pneumonia was apparent microscopically by day 2 and radiographically and grossly apparent by day 7 post infection (PI). Early lesions of bronchopneumonia soon progressed to more severe bronchointerstitial pneumonia with pyogranuloma formation. Extrapulmonary dissemination appeared to be a function of pyogranuloma invasion of pulmonary vasculature, which peaked around day 7 PI. Histopathology indicated that leukocytoclastic vasculitis was the central step in dissemination of B. pseudomallei from the lungs as well as in the establishment of new lesions. While higher doses of organism in goats can produce acute fatal disease, the dose investigated and resulting disease had many similarities to human melioidosis and may warrant further development to provide a model for the study of both natural and bioterrorism associated disease. Public Library of Science 2012-08-15 /pmc/articles/PMC3419728/ /pubmed/22916225 http://dx.doi.org/10.1371/journal.pone.0043207 Text en © 2012 Soffler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Soffler, Carl
Bosco-Lauth, Angela M.
Aboellail, Tawfik A.
Marolf, Angela J.
Bowen, Richard A.
Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis
title Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis
title_full Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis
title_fullStr Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis
title_full_unstemmed Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis
title_short Development and Characterization of a Caprine Aerosol Infection Model of Melioidosis
title_sort development and characterization of a caprine aerosol infection model of melioidosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419728/
https://www.ncbi.nlm.nih.gov/pubmed/22916225
http://dx.doi.org/10.1371/journal.pone.0043207
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