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Rho GTPase Expression in Human Myeloid Cells
Myeloid cells are critical for innate immunity and the initiation of adaptive immunity. Strict regulation of the adhesive and migratory behavior is essential for proper functioning of these cells. Rho GTPases are important regulators of adhesion and migration; however, it is unknown which Rho GTPase...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420873/ https://www.ncbi.nlm.nih.gov/pubmed/22916134 http://dx.doi.org/10.1371/journal.pone.0042563 |
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author | van Helden, Suzanne F. G. Anthony, Eloise C. Dee, Rob Hordijk, Peter L. |
author_facet | van Helden, Suzanne F. G. Anthony, Eloise C. Dee, Rob Hordijk, Peter L. |
author_sort | van Helden, Suzanne F. G. |
collection | PubMed |
description | Myeloid cells are critical for innate immunity and the initiation of adaptive immunity. Strict regulation of the adhesive and migratory behavior is essential for proper functioning of these cells. Rho GTPases are important regulators of adhesion and migration; however, it is unknown which Rho GTPases are expressed in different myeloid cells. Here, we use a qPCR-based approach to investigate Rho GTPase expression in myeloid cells. We found that the mRNAs encoding Cdc42, RhoQ, Rac1, Rac2, RhoA and RhoC are the most abundant. In addition, RhoG, RhoB, RhoF and RhoV are expressed at low levels or only in specific cell types. More differentiated cells along the monocyte-lineage display lower levels of Cdc42 and RhoV, while RhoC mRNA is more abundant. In addition, the Rho GTPase expression profile changes during dendritic cell maturation with Rac1 being upregulated and Rac2 downregulated. Finally, GM-CSF stimulation, during macrophage and osteoclast differentiation, leads to high expression of Rac2, while M-CSF induces high levels of RhoA, showing that these cytokines induce a distinct pattern. Our data uncover cell type specific modulation of the Rho GTPase expression profile in hematopoietic stem cells and in more differentiated cells of the myeloid lineage. |
format | Online Article Text |
id | pubmed-3420873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34208732012-08-22 Rho GTPase Expression in Human Myeloid Cells van Helden, Suzanne F. G. Anthony, Eloise C. Dee, Rob Hordijk, Peter L. PLoS One Research Article Myeloid cells are critical for innate immunity and the initiation of adaptive immunity. Strict regulation of the adhesive and migratory behavior is essential for proper functioning of these cells. Rho GTPases are important regulators of adhesion and migration; however, it is unknown which Rho GTPases are expressed in different myeloid cells. Here, we use a qPCR-based approach to investigate Rho GTPase expression in myeloid cells. We found that the mRNAs encoding Cdc42, RhoQ, Rac1, Rac2, RhoA and RhoC are the most abundant. In addition, RhoG, RhoB, RhoF and RhoV are expressed at low levels or only in specific cell types. More differentiated cells along the monocyte-lineage display lower levels of Cdc42 and RhoV, while RhoC mRNA is more abundant. In addition, the Rho GTPase expression profile changes during dendritic cell maturation with Rac1 being upregulated and Rac2 downregulated. Finally, GM-CSF stimulation, during macrophage and osteoclast differentiation, leads to high expression of Rac2, while M-CSF induces high levels of RhoA, showing that these cytokines induce a distinct pattern. Our data uncover cell type specific modulation of the Rho GTPase expression profile in hematopoietic stem cells and in more differentiated cells of the myeloid lineage. Public Library of Science 2012-08-16 /pmc/articles/PMC3420873/ /pubmed/22916134 http://dx.doi.org/10.1371/journal.pone.0042563 Text en © 2012 van Helden et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article van Helden, Suzanne F. G. Anthony, Eloise C. Dee, Rob Hordijk, Peter L. Rho GTPase Expression in Human Myeloid Cells |
title | Rho GTPase Expression in Human Myeloid Cells |
title_full | Rho GTPase Expression in Human Myeloid Cells |
title_fullStr | Rho GTPase Expression in Human Myeloid Cells |
title_full_unstemmed | Rho GTPase Expression in Human Myeloid Cells |
title_short | Rho GTPase Expression in Human Myeloid Cells |
title_sort | rho gtpase expression in human myeloid cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420873/ https://www.ncbi.nlm.nih.gov/pubmed/22916134 http://dx.doi.org/10.1371/journal.pone.0042563 |
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