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AhNRAMP1 iron transporter is involved in iron acquisition in peanut

Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to diva...

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Autores principales: Xiong, Hongchun, Kobayashi, Takanori, Kakei, Yusuke, Senoura, Takeshi, Nakazono, Mikio, Takahashi, Hirokazu, Nakanishi, Hiromi, Shen, Hongyun, Duan, Penggen, Guo, Xiaotong, Nishizawa, Naoko K., Zuo, Yuanmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3421984/
https://www.ncbi.nlm.nih.gov/pubmed/22611231
http://dx.doi.org/10.1093/jxb/ers117
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author Xiong, Hongchun
Kobayashi, Takanori
Kakei, Yusuke
Senoura, Takeshi
Nakazono, Mikio
Takahashi, Hirokazu
Nakanishi, Hiromi
Shen, Hongyun
Duan, Penggen
Guo, Xiaotong
Nishizawa, Naoko K.
Zuo, Yuanmei
author_facet Xiong, Hongchun
Kobayashi, Takanori
Kakei, Yusuke
Senoura, Takeshi
Nakazono, Mikio
Takahashi, Hirokazu
Nakanishi, Hiromi
Shen, Hongyun
Duan, Penggen
Guo, Xiaotong
Nishizawa, Naoko K.
Zuo, Yuanmei
author_sort Xiong, Hongchun
collection PubMed
description Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to divalent metal transporters of the natural resistance-associated macrophage protein (NRAMP) gene family was isolated. Yeast complementation assays suggested that AhNRAMP1 encodes a functional iron transporter. Moreover, the mRNA level of AhNRAMP1 was obviously induced by iron deficiency in both roots and leaves. Transient expression, laser microdissection, and in situ hybridization analyses revealed that AhNRAMP1 was mainly localized on the plasma membrane of the epidermis of peanut roots. Induced expression of AhNRAMP1 in tobacco conferred enhanced tolerance to iron deprivation. These results suggest that the AhNRAMP1 is possibly involved in iron acquisition in peanut plants.
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spelling pubmed-34219842012-08-17 AhNRAMP1 iron transporter is involved in iron acquisition in peanut Xiong, Hongchun Kobayashi, Takanori Kakei, Yusuke Senoura, Takeshi Nakazono, Mikio Takahashi, Hirokazu Nakanishi, Hiromi Shen, Hongyun Duan, Penggen Guo, Xiaotong Nishizawa, Naoko K. Zuo, Yuanmei J Exp Bot Research Paper Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to divalent metal transporters of the natural resistance-associated macrophage protein (NRAMP) gene family was isolated. Yeast complementation assays suggested that AhNRAMP1 encodes a functional iron transporter. Moreover, the mRNA level of AhNRAMP1 was obviously induced by iron deficiency in both roots and leaves. Transient expression, laser microdissection, and in situ hybridization analyses revealed that AhNRAMP1 was mainly localized on the plasma membrane of the epidermis of peanut roots. Induced expression of AhNRAMP1 in tobacco conferred enhanced tolerance to iron deprivation. These results suggest that the AhNRAMP1 is possibly involved in iron acquisition in peanut plants. Oxford University Press 2012-07 2012-05-18 /pmc/articles/PMC3421984/ /pubmed/22611231 http://dx.doi.org/10.1093/jxb/ers117 Text en © 2012 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Paper
Xiong, Hongchun
Kobayashi, Takanori
Kakei, Yusuke
Senoura, Takeshi
Nakazono, Mikio
Takahashi, Hirokazu
Nakanishi, Hiromi
Shen, Hongyun
Duan, Penggen
Guo, Xiaotong
Nishizawa, Naoko K.
Zuo, Yuanmei
AhNRAMP1 iron transporter is involved in iron acquisition in peanut
title AhNRAMP1 iron transporter is involved in iron acquisition in peanut
title_full AhNRAMP1 iron transporter is involved in iron acquisition in peanut
title_fullStr AhNRAMP1 iron transporter is involved in iron acquisition in peanut
title_full_unstemmed AhNRAMP1 iron transporter is involved in iron acquisition in peanut
title_short AhNRAMP1 iron transporter is involved in iron acquisition in peanut
title_sort ahnramp1 iron transporter is involved in iron acquisition in peanut
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3421984/
https://www.ncbi.nlm.nih.gov/pubmed/22611231
http://dx.doi.org/10.1093/jxb/ers117
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