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Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations

BACKGROUND: Niemann-pick C1-like 1 (NPC1L1) is a key protein for intestinal cholesterol transportation. Common single nucleotide polymorphisms (SNPs) in the NPC1L1 gene have been associated with cholesterol absorption and serum lipid levels. The present study was undertaken to explore the possible a...

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Autores principales: Miao, Lin, Yin, Rui-Xing, Hu, Xi-Jiang, Wu, Dong-Feng, Cao, Xiao-Li, Li, Qing, Yan, Ting-Ting, Aung, Lynn Htet Htet, Wu, Jin-Zhen, Lin, Wei-Xiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422998/
https://www.ncbi.nlm.nih.gov/pubmed/22646906
http://dx.doi.org/10.1186/1476-511X-11-61
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author Miao, Lin
Yin, Rui-Xing
Hu, Xi-Jiang
Wu, Dong-Feng
Cao, Xiao-Li
Li, Qing
Yan, Ting-Ting
Aung, Lynn Htet Htet
Wu, Jin-Zhen
Lin, Wei-Xiong
author_facet Miao, Lin
Yin, Rui-Xing
Hu, Xi-Jiang
Wu, Dong-Feng
Cao, Xiao-Li
Li, Qing
Yan, Ting-Ting
Aung, Lynn Htet Htet
Wu, Jin-Zhen
Lin, Wei-Xiong
author_sort Miao, Lin
collection PubMed
description BACKGROUND: Niemann-pick C1-like 1 (NPC1L1) is a key protein for intestinal cholesterol transportation. Common single nucleotide polymorphisms (SNPs) in the NPC1L1 gene have been associated with cholesterol absorption and serum lipid levels. The present study was undertaken to explore the possible association of NPC1L1 rs2072183 1735 C > G SNP and several environmental factors with serum lipid levels in the Mulao and Han populations. METHODS: Genotyping of the rs2072183 SNP was performed in 688 subjects of Mulao and 738 participants of Han Chinese. The interactions between NPC1L1 1735 C > G polymorphism and several environmental factors on serum lipid phenotypes were tested using the factorial design covariance analysis after controlling for potential confounders. RESULTS: The frequency of G allele was lower in Mulao than in Han (29.72% vs. 37.26%, P < 0.001). The frequency of CC, CG and GG genotypes was 49.85%, 40.84% and 9.31% in Mulao, and 39.30%, 46.88% and 13.82% in Han (P < 0.001); respectively. The levels of low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) B and the ratio of ApoAI/ApoB in Han but not in Mulao were different among the three genotypes (P < 0.05 for all), the subjects with GG and CG genotypes had higher LDL-C, ApoB levels and lower ApoAI/ApoB ratio than the subjects with CC genotype. Subgroup analysis showed that the G allele carriers in Han had higher total cholesterol (TC), LDL-C and ApoB levels in males (P < 0.05) and lower ApoAI/ApoB ratio in both sexes (P < 0.05) than the G allele noncarriers. The G allele carriers in Mulao had higher TC and LDL-C levels in males (P < 0.05) and lower high-density lipoprotein cholesterol (HDL-C) levels in both sexes (P < 0.05) than the G allele noncarriers. Serum TC, LDL-C, ApoB levels and ApoAI/ApoB ratio were correlated with genotypes in Han males (P < 0.05) but not in females. Serum lipid parameters were also correlated with several environmental factors. The genotypes of rs2072183 SNP were interacted with gender or cigarette smoking to influence serum TC and HDL-C levels in Mulao, whereas the genotypes of rs2072183 SNP were interacted with several environmental factors to influence all seven lipid traits in Han (P < 0.05-0.01). CONCLUSIONS: The present study suggests that the rs2072183 SNP in NPC1L1 gene and its association with serum lipid profiles are different between the Mulao and Han populations. The difference in serum lipid profiles between the two ethnic groups might partly result from different rs2072183 SNP or NPC1L1 gene-environmental interactions.
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spelling pubmed-34229982012-08-21 Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations Miao, Lin Yin, Rui-Xing Hu, Xi-Jiang Wu, Dong-Feng Cao, Xiao-Li Li, Qing Yan, Ting-Ting Aung, Lynn Htet Htet Wu, Jin-Zhen Lin, Wei-Xiong Lipids Health Dis Research BACKGROUND: Niemann-pick C1-like 1 (NPC1L1) is a key protein for intestinal cholesterol transportation. Common single nucleotide polymorphisms (SNPs) in the NPC1L1 gene have been associated with cholesterol absorption and serum lipid levels. The present study was undertaken to explore the possible association of NPC1L1 rs2072183 1735 C > G SNP and several environmental factors with serum lipid levels in the Mulao and Han populations. METHODS: Genotyping of the rs2072183 SNP was performed in 688 subjects of Mulao and 738 participants of Han Chinese. The interactions between NPC1L1 1735 C > G polymorphism and several environmental factors on serum lipid phenotypes were tested using the factorial design covariance analysis after controlling for potential confounders. RESULTS: The frequency of G allele was lower in Mulao than in Han (29.72% vs. 37.26%, P < 0.001). The frequency of CC, CG and GG genotypes was 49.85%, 40.84% and 9.31% in Mulao, and 39.30%, 46.88% and 13.82% in Han (P < 0.001); respectively. The levels of low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) B and the ratio of ApoAI/ApoB in Han but not in Mulao were different among the three genotypes (P < 0.05 for all), the subjects with GG and CG genotypes had higher LDL-C, ApoB levels and lower ApoAI/ApoB ratio than the subjects with CC genotype. Subgroup analysis showed that the G allele carriers in Han had higher total cholesterol (TC), LDL-C and ApoB levels in males (P < 0.05) and lower ApoAI/ApoB ratio in both sexes (P < 0.05) than the G allele noncarriers. The G allele carriers in Mulao had higher TC and LDL-C levels in males (P < 0.05) and lower high-density lipoprotein cholesterol (HDL-C) levels in both sexes (P < 0.05) than the G allele noncarriers. Serum TC, LDL-C, ApoB levels and ApoAI/ApoB ratio were correlated with genotypes in Han males (P < 0.05) but not in females. Serum lipid parameters were also correlated with several environmental factors. The genotypes of rs2072183 SNP were interacted with gender or cigarette smoking to influence serum TC and HDL-C levels in Mulao, whereas the genotypes of rs2072183 SNP were interacted with several environmental factors to influence all seven lipid traits in Han (P < 0.05-0.01). CONCLUSIONS: The present study suggests that the rs2072183 SNP in NPC1L1 gene and its association with serum lipid profiles are different between the Mulao and Han populations. The difference in serum lipid profiles between the two ethnic groups might partly result from different rs2072183 SNP or NPC1L1 gene-environmental interactions. BioMed Central 2012-05-30 /pmc/articles/PMC3422998/ /pubmed/22646906 http://dx.doi.org/10.1186/1476-511X-11-61 Text en Copyright ©2012 Miao et al.; licensee BioMed Central Ltd http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Miao, Lin
Yin, Rui-Xing
Hu, Xi-Jiang
Wu, Dong-Feng
Cao, Xiao-Li
Li, Qing
Yan, Ting-Ting
Aung, Lynn Htet Htet
Wu, Jin-Zhen
Lin, Wei-Xiong
Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations
title Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations
title_full Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations
title_fullStr Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations
title_full_unstemmed Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations
title_short Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations
title_sort association of rs2072183 snp and serum lipid levels in the mulao and han populations
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422998/
https://www.ncbi.nlm.nih.gov/pubmed/22646906
http://dx.doi.org/10.1186/1476-511X-11-61
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