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Isolation of Plasmodium falciparum by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples
BACKGROUND: Flow cytometry and cell sorting are powerful tools enabling the selection of particular cell types within heterogeneous cell mixtures. These techniques, combined with whole genome amplification that non-specifically amplify small amounts of starting DNA, offer exciting new opportunities...
Autores principales: | Boissière, Anne, Arnathau, Céline, Duperray, Christophe, Berry, Laurence, Lachaud, Laurence, Renaud, François, Durand, Patrick, Prugnolle, Franck |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423005/ https://www.ncbi.nlm.nih.gov/pubmed/22583664 http://dx.doi.org/10.1186/1475-2875-11-163 |
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